Salt-dependent regulation of archaellins in Haloarcula marismortui<br>

Abstract: Microorganisms require a motility structure to move towards optimal growth condi-tions. The motility structure from archaea, the archaellum, is fundamentally different from its bacterial counterpart, the flagellum, and is assembled in a similar fashion as type IV pili. The archaellum filament consists of thousands of copies of N-terminally processed archaellin proteins. Several archaea, such as the euryarchaeon Haloarcula marismortui, encode multiple archaellins. Two archaellins of H. marismortui display differential stability under various ionic strengths. This suggests that these proteins behave as ecoparalogs and perform the same function under different environmen-tal conditions. Here, we explored this intriguing system to study the differential regu-lation of these ecoparalogous archaellins by monitoring their transcription, translation, and assembly into filaments. The salt concentration of the growth medium induced differential expression of the two archaellins. In addition, this analysis indicated that archaellation in H. marismortui is majorly regulated on the level of secretion, by a still unknown mechanism. These findings indicate that in archaea, multiple encoded ar-chaellins are not completely redundant, but in fact can display subtle functional dif-ferences, which enable cells to cope with varying environmental conditions

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: MicrobiologyOpen. - 8, 5 (2019) , e00718, ISSN: 2045-8827

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2019
• Creator: Syutkin, Alexey S. ; Wolferen, Marleen van ; Surin, Alexey K. ; Albers, Sonja Verena ; Pyatibratov, Mikhail G. ; Fedorov, Oleg V. ; Quax, Tessa
• Contributor: Molekulare Biologie der Archaeen

Further information

• DOI: 10.1002/mbo3.718
• URN: urn:nbn:de:bsz:25-freidok-1489795
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 25.03.2025, 1:56 PM CET