Designed miniaturization of microfluidic biosensor platforms using the stop-flow technique

Abstract: Here, we present a novel approach to increase the degree of miniaturization as well as the sensitivity of biosensor platforms by the optimization of microfluidic stop-flow techniques independent of the applied detection technique (e.g. electrochemical or optical). The readout of the labeled bioassays, immobilized in a microfluidic channel, under stop-flow conditions leads to a rectangular shaped peak signal. Data evaluation using the peak height allows for a high level miniaturization of the channel geometries. To study the main advantages and limitations of this method by numerical simulations, a universally applicable model system is introduced for the first time. Consequently, proof-of-principle experiments were successfully performed with standard and miniaturized versions of an electrochemical biosensor platform utilizing a repressor protein-based assay for tetracycline antibiotics. Herein, the measured current peak heights are the same despite the sextuple reduction of the channel dimensions. Thus, this results in a 22-fold signal amplification compared to the constant flow measurements in the case of the miniaturized version

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch
• Anmerkungen: Analyst. 141 (2016), article id 6073. DOI 10.1039/C6AN01330A, issn: 1364-5528 ; IN COPYRIGHT http://rightsstatements.org/page/InC/1.0 rs

Klassifikation und Themen

• Klassifikation: Biowissenschaften, Biologie
• Schlagwort: Mikrofluidik ; Elektrochemie ; Amperometrie ; Antibiotikum ; Tetracycline

Beteiligte, Orts- und Zeitangaben

• Ereignis: Veröffentlichung
• (wo): Freiburg
• (wer): Universität
• (wann): 2016
• Urheber: Dincer, Can ; Kling, André ; Chatelle, Claire ; Armbrecht, Lucas ; Kieninger, Jochen ; Weber, Wilfried ; Urban, Gerald A.
• Beteiligte Personen und Organisationen: Freiburger Materialforschungszentrum ; Institut für Mikrosystemtechnik ; BIOSS Centre for Biological Signalling Studies ; Fakultät für Biologie ; Spemann Graduate School of Biology and Medicine (SGBM), Albert Ludwigs University Freiburg, Freiburg, Germany ; Technische Fakultät ; Albert-Ludwigs-Universität Freiburg

Weitere Informationen

• DOI: 10.1039/C6AN01330A
• URN: urn:nbn:de:bsz:25-freidok-118122
• Rechteinformation: Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 14.08.2025, 10:46 MESZ