Biological mechanisms of neurostimulation: An in situ hybridization based study comparing Deep Brain Stimulation and Optogenetic stimulation of the Medial Forebrain Bundle
Abstract: Aims: Studying the impact of different stimulation times and parameters on of the expression of key biological markers following unilateral optical stimulation of midbrain dopaminergic pathways or non-selective electrical Deep Brain Stimulation (DBS) of the medial forebrain bundle (MFB).
Methods: TH-cre rats either received i.) Unilateral AAV Virus injection in the ventral tegmental area (VTA) and optical fibers implanted in MFB (Opto-group); or ii.) Unilateral electrode implantation in the MFB (DBS-group). Brain tissues from selected areas of interest were recovered and in situ hybridization was carried out for key biological markers.
Results: Despite the unilateral stimulation, most of the changes in the expression levels occurred bilaterally, and the stimulation affected the biomarkers differently in different regions. Stimulation with both modalities increased gene expression, although more so with DBS compared to optogenetic stimulation. Increased stimulation sessions, i.e. 7 daily stimulations versus a single stimulation, did not translate into increased expression in most cases, and neither did the frequency (30 vs 130Hz) have a clear- cut effect, if anything, 30Hz increased gene expression more.
Conclusions: Unilateral stimulation can impact gene expression in both hemispheres, but differences were observed across stimulation frequencies. The stimulation induced differences in patterns of gene expression changes were interpreted in the context of stimulation modality, parameters and MFB connectivity
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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Universität Freiburg, Dissertation, 2020
- Schlagwort
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Brain stimulation
Hybridization
Prosencephalon
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2020
- Urheber
- Beteiligte Personen und Organisationen
- DOI
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10.6094/UNIFR/167559
- URN
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urn:nbn:de:bsz:25-freidok-1675596
- Rechteinformation
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- Letzte Aktualisierung
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14.08.2025, 10:54 MESZ
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- 2020
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