Sensor access to the cellular microenvironment using the sensing cell culture flask
Abstract: The Sensing Cell Culture Flask (SCCF) is a cell culture monitoring system accessing the cellular microenvironment in 2D cell culture using electrochemical microsensors. The system is based on microfabricated sensor chips embedded in standard cell culture flasks. Ideally, the sensor chips could be equipped with any electrochemical sensor. Its transparency allows optical inspection of the cells during measurement. The surface of the sensor chip is in-plane with the flask surface allowing undisturbed cell growth on the sensor chip. A custom developed rack system allows easy usage of multiple flasks in parallel within an incubator. The presented data demonstrates the application of the SCCF with brain tumor (T98G) and breast cancer (T-47D) cells. Amperometric oxygen sensors were used to monitor cellular respiration with different incubation conditions. Cellular acidification was accessed with potentiometric pH sensors using electrodeposited iridium oxide films. The system itself provides the foundation for electrochemical monitoring systems in 3D cell culture
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Classification
-
Industrielle und handwerkliche Fertigung
- Keyword
-
Mikrosensor
Sauerstoff
Wasserstoffionenkonzentration
Hypoxie
- Event
-
Veröffentlichung
- (where)
-
Freiburg
- (who)
-
Universität
- (when)
-
2018
- Creator
-
Kieninger, Jochen
Tamari, Yaara
Enderle, Barbara
Jobst, Gerhard
Sandvik, Joe A.
Pettersen, Erik O.
Urban, Gerald A.
- DOI
-
10.3390/bios8020044
- URN
-
urn:nbn:de:bsz:25-freidok-161876
- Rights
-
Kein Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
25.03.2025, 1:48 PM CET
Data provider
This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Kieninger, Jochen
- Tamari, Yaara
- Enderle, Barbara
- Jobst, Gerhard
- Sandvik, Joe A.
- Pettersen, Erik O.
- Urban, Gerald A.
- Universität
Time of origin
- 2018
Other Objects (12)
Investigations of metal-organic frameworks and metal nanoparticle functionalised tungsten oxide for gas sensing applications : = Untersuchung von Metal-organischen Gerüsten und mit metallischen Nanopartikeln funktionalisiertem Wolframoxid für Gasdetektionsanwendungen
Utilizing the ability of electrochemical NO sensors: micro sensor designs for hypoxic cell cultures : = Designs für Mikrosensoren für hypoxische Zellkulturen unter Ausnutzung der Leistungsfähigkeit von elektrochemischen NO-Sensoren
Characterization of the adsorption of biomolecules in open electrowetting on dielectrics microfluidics-tool platform for bio-analytical applications : = Charakterisierung des Adsorptionsverhaltens von Biomolekülen auf Open Electrowetting On Dielectrics Mikrofluidikplattform für Bioanalytische Anwendungen
Electrochemical microfluidic multiplexed biosensor platform for point-of-care testing
Microfluidic impedance biosensors for monitoring cancer cell behaviors
Multiparametric, flexible microsensors for in vivo application
Electrochemical microsensors for superoxide monitoring in cell culture : = Elektrochemische Mikrosensoren fuer Superoxid-Monitoring in Zellkultur
Electrochemical microsensor system for cell culture monitoring : = Elektrochemisches Mikrosensor-System zum Zellkultur-Monitoring
Microfluidic immunosensor for point-of-care diagnostics : = Microfluidischer Immunosensor für patientennahe Diagnostik
Investigation of a magnetron enhanced AF plasma polymerization process for sensor coating : = Untersuchung eines Magnetron-Angereicherten AF Plasma-Polymerisationsprozesses für Sensorbeschichtung
Fabrication of interdigitated electrode arrays for biosensors by advanced mask aligner lithography
A microfluidic dual chip system for rapid pathogen detection
Investigations of metal-organic frameworks and metal nanoparticle functionalised tungsten oxide for gas sensing applications : = Untersuchung von Metal-organischen Gerüsten und mit metallischen Nanopartikeln funktionalisiertem Wolframoxid für Gasdetektionsanwendungen
Utilizing the ability of electrochemical NO sensors: micro sensor designs for hypoxic cell cultures : = Designs für Mikrosensoren für hypoxische Zellkulturen unter Ausnutzung der Leistungsfähigkeit von elektrochemischen NO-Sensoren
Characterization of the adsorption of biomolecules in open electrowetting on dielectrics microfluidics-tool platform for bio-analytical applications : = Charakterisierung des Adsorptionsverhaltens von Biomolekülen auf Open Electrowetting On Dielectrics Mikrofluidikplattform für Bioanalytische Anwendungen
Electrochemical microfluidic multiplexed biosensor platform for point-of-care testing
Microfluidic impedance biosensors for monitoring cancer cell behaviors
Multiparametric, flexible microsensors for in vivo application
Electrochemical microsensors for superoxide monitoring in cell culture : = Elektrochemische Mikrosensoren fuer Superoxid-Monitoring in Zellkultur
Electrochemical microsensor system for cell culture monitoring : = Elektrochemisches Mikrosensor-System zum Zellkultur-Monitoring
Microfluidic immunosensor for point-of-care diagnostics : = Microfluidischer Immunosensor für patientennahe Diagnostik
Investigation of a magnetron enhanced AF plasma polymerization process for sensor coating : = Untersuchung eines Magnetron-Angereicherten AF Plasma-Polymerisationsprozesses für Sensorbeschichtung
Fabrication of interdigitated electrode arrays for biosensors by advanced mask aligner lithography
A microfluidic dual chip system for rapid pathogen detection
Investigations of metal-organic frameworks and metal nanoparticle functionalised tungsten oxide for gas sensing applications : = Untersuchung von Metal-organischen Gerüsten und mit metallischen Nanopartikeln funktionalisiertem Wolframoxid für Gasdetektionsanwendungen
Utilizing the ability of electrochemical NO sensors: micro sensor designs for hypoxic cell cultures : = Designs für Mikrosensoren für hypoxische Zellkulturen unter Ausnutzung der Leistungsfähigkeit von elektrochemischen NO-Sensoren
Characterization of the adsorption of biomolecules in open electrowetting on dielectrics microfluidics-tool platform for bio-analytical applications : = Charakterisierung des Adsorptionsverhaltens von Biomolekülen auf Open Electrowetting On Dielectrics Mikrofluidikplattform für Bioanalytische Anwendungen
Electrochemical microfluidic multiplexed biosensor platform for point-of-care testing
Microfluidic impedance biosensors for monitoring cancer cell behaviors
Multiparametric, flexible microsensors for in vivo application
Electrochemical microsensors for superoxide monitoring in cell culture : = Elektrochemische Mikrosensoren fuer Superoxid-Monitoring in Zellkultur
Electrochemical microsensor system for cell culture monitoring : = Elektrochemisches Mikrosensor-System zum Zellkultur-Monitoring
Microfluidic immunosensor for point-of-care diagnostics : = Microfluidischer Immunosensor für patientennahe Diagnostik
Investigation of a magnetron enhanced AF plasma polymerization process for sensor coating : = Untersuchung eines Magnetron-Angereicherten AF Plasma-Polymerisationsprozesses für Sensorbeschichtung
Fabrication of interdigitated electrode arrays for biosensors by advanced mask aligner lithography