Artificial Cytoskeleton Assembly for Synthetic Cell Motility
Abstract: Imitation of cellular processes in cell‐like compartments is a current research focus in synthetic biology. Here, a method is introduced for assembling an artificial cytoskeleton in a synthetic cell model system based on a poly (N‐isopropyl acrylamide) (PNIPAM) composite material. Toward this end, a PNIPAM‐based composite material inside water‐in‐oil droplets that are stabilized with PNIPAM‐functionalized and commercial fluorosurfactants is introduced. The temperature‐mediated contraction/release behavior of the PNIPAM‐based cytoskeleton is investigated. The reversibility of the PNIPAM transition is further examined in bulk and in droplets and it could be shown that hydrogel induced deformation could be used to controllably manipulate droplet‐based synthetic cell motility upon temperature changes. It is envisioned that a combination of the presented artificial cytoskeleton with naturally occurring components might expand the bandwidth of the bottom‐up synthetic biology.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Artificial Cytoskeleton Assembly for Synthetic Cell Motility ; day:16 ; month:12 ; year:2022 ; extent:10
Macromolecular bioscience ; (16.12.2022) (gesamt 10)
- Creator
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Sauter, Désirée
Schröter, Martin
Frey, Christoph
Weber, Cornelia
Mersdorf, Ulrike
Janiesch, Jan‐Willi
Platzman, Ilia
Spatz, Joachim P.
- DOI
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10.1002/mabi.202200437
- URN
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urn:nbn:de:101:1-2022121714170033697476
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:24 AM CEST
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Associated
- Sauter, Désirée
- Schröter, Martin
- Frey, Christoph
- Weber, Cornelia
- Mersdorf, Ulrike
- Janiesch, Jan‐Willi
- Platzman, Ilia
- Spatz, Joachim P.
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Development of functional droplet based microfluidic systems for synthetic biology and biomedical high-throughput applications