Artificial Cytoskeleton Assembly for Synthetic Cell Motility
Abstract: Imitation of cellular processes in cell‐like compartments is a current research focus in synthetic biology. Here, a method is introduced for assembling an artificial cytoskeleton in a synthetic cell model system based on a poly (N‐isopropyl acrylamide) (PNIPAM) composite material. Toward this end, a PNIPAM‐based composite material inside water‐in‐oil droplets that are stabilized with PNIPAM‐functionalized and commercial fluorosurfactants is introduced. The temperature‐mediated contraction/release behavior of the PNIPAM‐based cytoskeleton is investigated. The reversibility of the PNIPAM transition is further examined in bulk and in droplets and it could be shown that hydrogel induced deformation could be used to controllably manipulate droplet‐based synthetic cell motility upon temperature changes. It is envisioned that a combination of the presented artificial cytoskeleton with naturally occurring components might expand the bandwidth of the bottom‐up synthetic biology.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Artificial Cytoskeleton Assembly for Synthetic Cell Motility ; day:16 ; month:12 ; year:2022 ; extent:10
Macromolecular bioscience ; (16.12.2022) (gesamt 10)
- Urheber
-
Sauter, Désirée
Schröter, Martin
Frey, Christoph
Weber, Cornelia
Mersdorf, Ulrike
Janiesch, Jan‐Willi
Platzman, Ilia
Spatz, Joachim P.
- DOI
-
10.1002/mabi.202200437
- URN
-
urn:nbn:de:101:1-2022121714170033697476
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:24 MESZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Sauter, Désirée
- Schröter, Martin
- Frey, Christoph
- Weber, Cornelia
- Mersdorf, Ulrike
- Janiesch, Jan‐Willi
- Platzman, Ilia
- Spatz, Joachim P.
Ähnliche Objekte (12)
PNIPAM-containing droplets: The route towards an artificial cytoskeleton assembly based on synthetic and natural components
Nanopatterns for the localisation and seperation of molecular functional units : monomolecular adhesion points and patterns for manipulation of biological cell mechanics
Kontrollierte Mineralisation in dispersen Phasen von Zweiblockcopolymeren
Bottom-Up Assembly of Synthetic Model Systems for Cellular Adhesion
Microfluidic Approaches for the Sequential Bottom-up Assembly of Droplet-based Minimal Synthetic Cells
The Reconstitution of Eukaryotic Architecture and Motility via Microfluidic Technology
Engineering Polarity in Bottom-Up Synthetic Cells
Bottom‐Up Assembly of Functional Intracellular Synthetic Organelles by Droplet‐Based Microfluidics
Development of Droplet-Based Microfluidics for Synthetic Biology Applications
Bottom-up Assembly of Functional Extracellular Vesicles – Implications for Synthetic Biology and Biomedical Applications
Development of functional droplet based microfluidic systems for synthetic biology and biomedical high-throughput applications
Development of next-generation fluorinated block copolymers and bottom-up assembly of biomimetic cell interaction modules
PNIPAM-containing droplets: The route towards an artificial cytoskeleton assembly based on synthetic and natural components
Nanopatterns for the localisation and seperation of molecular functional units : monomolecular adhesion points and patterns for manipulation of biological cell mechanics
Kontrollierte Mineralisation in dispersen Phasen von Zweiblockcopolymeren
Bottom-Up Assembly of Synthetic Model Systems for Cellular Adhesion
Microfluidic Approaches for the Sequential Bottom-up Assembly of Droplet-based Minimal Synthetic Cells
The Reconstitution of Eukaryotic Architecture and Motility via Microfluidic Technology
Engineering Polarity in Bottom-Up Synthetic Cells
Bottom‐Up Assembly of Functional Intracellular Synthetic Organelles by Droplet‐Based Microfluidics
Development of Droplet-Based Microfluidics for Synthetic Biology Applications
Bottom-up Assembly of Functional Extracellular Vesicles – Implications for Synthetic Biology and Biomedical Applications
Development of functional droplet based microfluidic systems for synthetic biology and biomedical high-throughput applications
Development of next-generation fluorinated block copolymers and bottom-up assembly of biomimetic cell interaction modules
PNIPAM-containing droplets: The route towards an artificial cytoskeleton assembly based on synthetic and natural components
Nanopatterns for the localisation and seperation of molecular functional units : monomolecular adhesion points and patterns for manipulation of biological cell mechanics
Kontrollierte Mineralisation in dispersen Phasen von Zweiblockcopolymeren
Bottom-Up Assembly of Synthetic Model Systems for Cellular Adhesion
Microfluidic Approaches for the Sequential Bottom-up Assembly of Droplet-based Minimal Synthetic Cells
The Reconstitution of Eukaryotic Architecture and Motility via Microfluidic Technology
Engineering Polarity in Bottom-Up Synthetic Cells
Bottom‐Up Assembly of Functional Intracellular Synthetic Organelles by Droplet‐Based Microfluidics
Development of Droplet-Based Microfluidics for Synthetic Biology Applications
Bottom-up Assembly of Functional Extracellular Vesicles – Implications for Synthetic Biology and Biomedical Applications
Development of functional droplet based microfluidic systems for synthetic biology and biomedical high-throughput applications