Self-assembled magnetic bead chains for sensitivity enhancement of microfluidic electrochemical biosensor platforms
Abstract: In this paper, we present a novel approach to enhance the sensitivity of microfluidic biosensor platforms with self-assembled magnetic bead chains. An adjustable, more than 5-fold sensitivity enhancement is achieved by introducing a magnetic field gradient along a microfluidic channel by means of a soft-magnetic lattice with a 350 μm spacing. The alternating magnetic field induces the self-assembly of the magnetic beads in chains or clusters and thus improves the perfusion and active contact between the analyte and the beads. The soft-magnetic lattices can be applied independent of the channel geometry or chip material to any microfluidic biosensing platform. At the same time, the bead-based approach achieves chip reusability and shortened measurement times. The bead chain properties and the maximum flow velocity for bead retention were validated by optical microscopy in a glass capillary. The magnetic actuation system was successfully validated with a biotin-streptavidin model assay on a low-cost electrochemical microfluidic chip, fabricated by dry-film photoresist technology (DFR). Labelling with glucose oxidase (GOx) permits rapid electrochemical detection of enzymatically produced H₂O₂
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Lab on a Chip. 15 (2015), 4314-4321, DOI 10.1039/C5LC00796H, issn: 1473-0189
IN COPYRIGHT http://rightsstatements.org/page/InC/1.0 rs
- Classification
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Industrielle und handwerkliche Fertigung
- Keyword
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Lab on a Chip
Mikrofluidik
Elektrochemie
Sensor
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Albert-Ludwigs-Universität Freiburg
- (when)
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2015
- Creator
- Contributor
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Freiburger Materialforschungszentrum
Institut für Mikrosystemtechnik
Albert-Ludwigs-Universität Freiburg
- DOI
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10.1039/C5LC00796H
- URN
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urn:nbn:de:bsz:25-freidok-121974
- Rights
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Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:53 AM CEST
Data provider
This object is provided by:
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Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Armbrecht, Lucas
- Dincer, Can
- Kling, André
- Horák, Josef
- Kieninger, Jochen
- Urban, Gerald A.
- Freiburger Materialforschungszentrum
- Institut für Mikrosystemtechnik
- Albert-Ludwigs-Universität Freiburg
Time of origin
- 2015
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