On the shoulders of giants - reaching for nitrogenase
Abstract: Only a single enzyme system—nitrogenase—carries out the conversion of atmospheric N2 into bioavailable ammonium, an essential prerequisite for all organismic life. The reduction of this inert substrate at ambient conditions poses unique catalytic challenges that strain our mechanistic understanding even after decades of intense research. Structural biology has added its part to this greater tapestry, and in this review, I provide a personal (and highly biased) summary of the parts of the story to which I had the privilege to contribute. It focuses on the crystallographic analysis of the three isoforms of nitrogenases at high resolution and the binding of ligands and inhibitors to the active-site cofactors of the enzyme. In conjunction with the wealth of available biochemical, biophysical, and spectroscopic data on the protein, this has led us to a mechanistic hypothesis based on an elementary mechanism of repetitive hydride formation and insertion
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Molecules. - 28, 24 (2023) , 7959, ISSN: 1420-3049
- DOI
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10.3390/molecules28247959
- URN
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urn:nbn:de:bsz:25-freidok-2419071
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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25.03.2025, 1:45 PM CET
Data provider
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Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Einsle, Oliver
- Universität
Time of origin
- 2023
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Crystallographic and mutational studies of bacterial cytochrome c peroxidases : = Kristallografische und Mutagenese-Studien an bakteriellen Cytochrom c Peroxidasesn
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Structure and function of cytochrome c nitrite reductase
Binuclear CuA formation and the influence of calcium ions on metal cluster maturation in recombinant nitrous oxide reductase from Shewanella denitrificans
Investigation of the protection mechanism of nitrogenase by the Shethna protein
Structural and functional characterization of the Na+-translocating NADH:quinone oxidoreductase from Vibrio cholerae and YihU of the sulfoglycolysis pathway : = Strukturelle und funktionelle Charakterisierung der Na+-translozierenden NADH:Ubichinon Oxidoreduktase aus Vibrio cholerae und YihU des Sulfoglycolysis-Stoffwechselweges
Structural Characterization of Landomycin O- and C-Glycosyltransferases
Crystal structure determination of a subfragment of the NADH:ubiquinone oxidoreductase from Aquifex aeolicus and functional reconstitution of a nitrate - nitrite transporter from Escherichia coli : = Kristallstruktur eines Subfragmentes der NADH:Ubichinon Oxidoreduktase aus Aquifex aeolicus und die funktionelle Rekonstitution eines Nitrat- Nitrit Transporters aus Escherichia coli
Crystallographic characterization of multiheme enzymes: cytochrome c nitrite reductase from Campylobacter jejuni and octaheme sulfite reductase from Wolinella succinogenes : = Kristallographische Charakterisierung von Multihäm-Enzymen: Cytochrom c Nitrit Reduktase aus Campylobacter jejuni und Oktahäm Sulfit Reduktase aus Wolinella succinogenes
Structural characterisation of nitrogen fixation by the enzyme nitrogenase of Azotobacter vinelandii
Structural characterization of the metal-binding ligands S100A8/S100A9 and S100B of the receptor for advanced glycation end products : = Strukturelle Charakterisierung der metallbindenden Liganden S100A8/S100A9 und S100B des Rezeptors für Advanced Glycation End Products
Structural investigations on the Fe(II)/α-ketoglutarate dependent dioxygenase PlaO1 from Streptomyces sp. Tü6071 : = Strukturelle Studien an der Eisen(II)/α-Ketoglutarat-abhängigen Dioxygenase PlaO1 aus Streptomyces sp. Tü6071
Crystallographic and mutational studies of bacterial cytochrome c peroxidases : = Kristallografische und Mutagenese-Studien an bakteriellen Cytochrom c Peroxidasesn
Structure of the Vanadium Nitrogenase of Azotobacter vinelandii and mechanistic insights into biological nitrogen fixation
Structure and function of cytochrome c nitrite reductase
Binuclear CuA formation and the influence of calcium ions on metal cluster maturation in recombinant nitrous oxide reductase from Shewanella denitrificans
Investigation of the protection mechanism of nitrogenase by the Shethna protein
Structural and functional characterization of the Na+-translocating NADH:quinone oxidoreductase from Vibrio cholerae and YihU of the sulfoglycolysis pathway : = Strukturelle und funktionelle Charakterisierung der Na+-translozierenden NADH:Ubichinon Oxidoreduktase aus Vibrio cholerae und YihU des Sulfoglycolysis-Stoffwechselweges
Structural Characterization of Landomycin O- and C-Glycosyltransferases
Crystal structure determination of a subfragment of the NADH:ubiquinone oxidoreductase from Aquifex aeolicus and functional reconstitution of a nitrate - nitrite transporter from Escherichia coli : = Kristallstruktur eines Subfragmentes der NADH:Ubichinon Oxidoreduktase aus Aquifex aeolicus und die funktionelle Rekonstitution eines Nitrat- Nitrit Transporters aus Escherichia coli
Crystallographic characterization of multiheme enzymes: cytochrome c nitrite reductase from Campylobacter jejuni and octaheme sulfite reductase from Wolinella succinogenes : = Kristallographische Charakterisierung von Multihäm-Enzymen: Cytochrom c Nitrit Reduktase aus Campylobacter jejuni und Oktahäm Sulfit Reduktase aus Wolinella succinogenes
Structural characterisation of nitrogen fixation by the enzyme nitrogenase of Azotobacter vinelandii
Structural characterization of the metal-binding ligands S100A8/S100A9 and S100B of the receptor for advanced glycation end products : = Strukturelle Charakterisierung der metallbindenden Liganden S100A8/S100A9 und S100B des Rezeptors für Advanced Glycation End Products
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