Enzymatic Fluoromethylation as a Tool for ATP‐Independent Ligation
Abstract: S‐adenosylmethionine‐dependent methyltransferases are involved in countless biological processes, including signal transduction, epigenetics, natural product biosynthesis, and detoxification. Only a handful of carboxylate methyltransferases have evolved to participate in amide bond formation. In this report we show that enzyme‐catalyzed F‐methylation of carboxylate substrates produces F‐methyl esters that readily react with N‐ or S‐nucleophiles under physiological conditions. We demonstrate the applicability of this approach to the synthesis of small amides, hydroxamates, and thioesters, as well as to site‐specific protein modification and native chemical ligation.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Enzymatic Fluoromethylation as a Tool for ATP‐Independent Ligation ; day:29 ; month:11 ; year:2023 ; extent:9
Angewandte Chemie / International edition. International edition ; (29.11.2023) (gesamt 9)
- Creator
- DOI
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10.1002/anie.202312104
- URN
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urn:nbn:de:101:1-2023113014114287860151
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:25 AM CEST
Data provider
This object is provided by:
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Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Peng, Jiaming
- Hughes, Gregory R.
- Müller, Manuel M.
- Seebeck, Florian Peter
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Enzymatic Fluoromethylation as a Tool for ATP‐Independent Ligation
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Monitoring enzymatic ATP hydrolysis by EPR spectroscopy
Template‐Directed Copying of RNA by Non‐enzymatic Ligation †
Template‐Directed Copying of RNA by Non‐enzymatic Ligation †
Template-independent enzymatic synthesis of RNA oligonucleotides
ATP-independent activation of natriuretic peptide receptors
Synthesis of artificial building blocks for sortase-mediated ligation and their enzymatic linkage
Reaction Engineering and Comparison of Electroenzymatic and Enzymatic ATP Regeneration Systems
Reaction Engineering and Comparison of Electroenzymatic and Enzymatic ATP Regeneration Systems
Different Enzymatic Processing of γ-Phosphoramidate and γ-Phosphoester-Modified ATP Analogues
Enzymatic Fluoromethylation as a Tool for ATP‐Independent Ligation
Enzymatic C‐to‐C Protein Ligation
Enzymatic C‐to‐C Protein Ligation
Monitoring enzymatic ATP hydrolysis by EPR spectroscopy
Template‐Directed Copying of RNA by Non‐enzymatic Ligation †
Template‐Directed Copying of RNA by Non‐enzymatic Ligation †
Template-independent enzymatic synthesis of RNA oligonucleotides
ATP-independent activation of natriuretic peptide receptors
Synthesis of artificial building blocks for sortase-mediated ligation and their enzymatic linkage
Reaction Engineering and Comparison of Electroenzymatic and Enzymatic ATP Regeneration Systems
Reaction Engineering and Comparison of Electroenzymatic and Enzymatic ATP Regeneration Systems