Template‐Directed Copying of RNA by Non‐enzymatic Ligation †
Abstract: The non‐enzymatic replication of the primordial genetic material is thought to have enabled the evolution of early forms of RNA‐based life. However, the replication of oligonucleotides long enough to encode catalytic functions is problematic due to the low efficiency of template copying with mononucleotides. We show that template‐directed ligation can assemble long RNAs from shorter oligonucleotides, which would be easier to replicate. The rate of ligation can be greatly enhanced by employing a 3′‐amino group at the 3′‐end of each oligonucleotide, in combination with an N‐alkyl imidazole organocatalyst. These modifications enable the copying of RNA templates by the multistep ligation of tetranucleotide building blocks, as well as the assembly of long oligonucleotides using short splint oligonucleotides. We also demonstrate the formation of long oligonucleotides inside model prebiotic vesicles, which suggests a potential route to the assembly of artificial cells capable of evolution.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Template‐Directed Copying of RNA by Non‐enzymatic Ligation † ; volume:132 ; number:36 ; year:2020 ; pages:15812-15817 ; extent:6
Angewandte Chemie ; 132, Heft 36 (2020), 15812-15817 (gesamt 6)
- Creator
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Zhou, Lijun
O'Flaherty, Derek K.
Szostak, Jack W.
- DOI
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10.1002/ange.202004934
- URN
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urn:nbn:de:101:1-2022052911490739119108
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:30 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Zhou, Lijun
- O'Flaherty, Derek K.
- Szostak, Jack W.
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Template‐Directed Copying of RNA by Non‐enzymatic Ligation †
Non-enzymatic post-translational modifications of bovine milk proteins
Non‐Enzymatic RNA Backbone Proofreading through Energy‐Dissipative Recycling
Non-enzymatic reactions in biogenesis of fungal natural products
Non‐Enzymatic Assembly of a Minimized RNA Polymerase Ribozyme
Chemical carcinogens in non-enzymatic cytosine deamination: 3-isocyanatoacrylonitrile
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Non-enzymatic role of SOD1 in intestinal stem cell growth