Non‐Enzymatic RNA Backbone Proofreading through Energy‐Dissipative Recycling
Abstract: Non‐enzymatic oligomerization of activated ribonucleotides leads to ribonucleic acids that contain a mixture of 2′,5′‐ and 3′,5′‐linkages, and overcoming this backbone heterogeneity has long been considered a major limitation to the prebiotic emergence of RNA. Herein, we demonstrate non‐enzymatic chemistry that progressively converts 2′,5′‐linkages into 3′,5′‐linkages through iterative degradation and repair. The energetic costs of this proofreading are met by the hydrolytic turnover of a phosphate activating agent and an acylating agent. With multiple rounds of this energy‐dissipative recycling, we show that all‐3′,5′‐linked duplex RNA can emerge from a backbone heterogeneous mixture, thereby delineating a route that could have driven RNA evolution on the early earth.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Non‐Enzymatic RNA Backbone Proofreading through Energy‐Dissipative Recycling ; volume:129 ; number:23 ; year:2017 ; pages:6663-6666 ; extent:4
Angewandte Chemie ; 129, Heft 23 (2017), 6663-6666 (gesamt 4)
- Creator
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Mariani, Angelica
Sutherland, John D.
- DOI
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10.1002/ange.201703169
- URN
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urn:nbn:de:101:1-2022091806120171367502
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:21 AM CEST
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Associated
- Mariani, Angelica
- Sutherland, John D.
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