Non‐Enzymatic Assembly of a Minimized RNA Polymerase Ribozyme
Abstract: Central to the “RNA world” hypothesis of the origin of life is the emergence of an RNA catalyst capable of RNA replication. However, possible replicase ribozymes are quite complex and were likely predated by simpler non‐enzymatic replication reactions. The templated polymerisation of phosphorimidazolide (Imp) activated ribonucleotides currently appears as the most tractable route to both generate and replicate short RNA oligomer pools from which a replicase could emerge. Herein we demonstrate the rapid assembly of complex ribozymes from such Imp‐activated RNA fragment pools. Specifically, we show assembly of a newly selected minimal RNA polymerase ribozyme variant (150 nt) by RNA templated ligation of 5’‐2‐methylimidazole‐activated RNA oligomers <30 nucleotides long. Our results provide support for the possibility that complex RNA structures could have emerged from pools of activated RNA oligomers and outlines a path for the transition from non‐enzymatic/chemical to enzymatic RNA replication.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Non‐Enzymatic Assembly of a Minimized RNA Polymerase Ribozyme ; volume:1 ; number:1-2 ; year:2019 ; pages:1-4 ; extent:4
ChemSystemsChem ; 1, Heft 1-2 (2019), 1-4 (gesamt 4)
- Urheber
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Wachowius, Falk
Holliger, Philipp
- DOI
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10.1002/syst.201900004
- URN
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urn:nbn:de:101:1-2022072107183611956996
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:24 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Wachowius, Falk
- Holliger, Philipp
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