ESIPT fluorescence turn-on sensors for detection of short chain inorganic polyphosphate in water
Abstract: We introduce two water-soluble excited state intramolecular proton transfer (ESIPT) based fluorescent turn-on probes responding to inorganic polyphosphates. These ESIPT probes enable specific detection of short-chain inorganic polyphosphates over a range of different condensed phosphates. The probes are weakly emissive in their off-state due to the blocking of ESIPT by Cu2+ coordination. Removal of the copper ion through decomplexation by the analyte accesses the on-state. The probes detect polyphosphates over other biologically occurring phosphates, pyrophosphate, and nucleotides such as ATP, ADP, GTP. An optimal fluorescence response is observed with the short-chain polyphosphate polyP8. Furthermore, the probe shows selectivity towards linear polyphosphates over cyclic metaphosphates. The rapid ‘turn-off–turn-on’ fluorescence responses upon consecutive addition of Cu2+ and polyP8 are reversible, further highlighting sensor performance in an aqueous environment. One of the sensors is then used to monitor polyP digestion by an exopolyphosphatase (PPX)
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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Organic & biomolecular chemistry. - 23, 6 (2025) , 1373-1379, ISSN: 1477-0539
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2025
- Urheber
- DOI
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10.1039/d4ob01926a
- URN
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urn:nbn:de:bsz:25-freidok-2613344
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:35 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Kanti Roy, Subhra
- Moser, Sandra
- Dürr-Mayer, Tobias
- Hinkelmann, Rahel
- Jessen, Henning Jacob
- Universität
Entstanden
- 2025
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