ESIPT fluorescence turn-on sensors for detection of short chain inorganic polyphosphate in water
Abstract: We introduce two water-soluble excited state intramolecular proton transfer (ESIPT) based fluorescent turn-on probes responding to inorganic polyphosphates. These ESIPT probes enable specific detection of short-chain inorganic polyphosphates over a range of different condensed phosphates. The probes are weakly emissive in their off-state due to the blocking of ESIPT by Cu2+ coordination. Removal of the copper ion through decomplexation by the analyte accesses the on-state. The probes detect polyphosphates over other biologically occurring phosphates, pyrophosphate, and nucleotides such as ATP, ADP, GTP. An optimal fluorescence response is observed with the short-chain polyphosphate polyP8. Furthermore, the probe shows selectivity towards linear polyphosphates over cyclic metaphosphates. The rapid ‘turn-off–turn-on’ fluorescence responses upon consecutive addition of Cu2+ and polyP8 are reversible, further highlighting sensor performance in an aqueous environment. One of the sensors is then used to monitor polyP digestion by an exopolyphosphatase (PPX)
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Organic & biomolecular chemistry. - 23, 6 (2025) , 1373-1379, ISSN: 1477-0539
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2025
- Creator
- DOI
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10.1039/d4ob01926a
- URN
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urn:nbn:de:bsz:25-freidok-2613344
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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2025-08-15T07:35:56+0200
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Kanti Roy, Subhra
- Moser, Sandra
- Dürr-Mayer, Tobias
- Hinkelmann, Rahel
- Jessen, Henning Jacob
- Universität
Time of origin
- 2025
Other Objects (12)
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Characterisation of family 2 polyphosphate kinases
An update on polyphosphate in vivo activities
Metabolic consequences of polyphosphate synthesis and imminent phosphate limitation
Investigations on the synthesis and degradation of polyphosphate in vitro and its correlation to magic spot nucleotides
The inositol pyrophosphate metabolism of Dictyostelium discoideum does not regulate inorganic polyphosphate (polyP) synthesis
Make or break: the thermodynamic equilibrium of polyphosphate kinase-catalysed reactions
Vtc5, a novel subunit of the vacuolar transporter chaperone complex, regulates polyphosphate synthesis and phosphate homeostasis in yeast
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Untersuchungen zur intrazellulären Freisetzung von Nucleosidphosphaten
Synthesis of bisdiphosphoinositol polyphosphate prometabolites
Characterisation of family 2 polyphosphate kinases
An update on polyphosphate in vivo activities
Metabolic consequences of polyphosphate synthesis and imminent phosphate limitation
Investigations on the synthesis and degradation of polyphosphate in vitro and its correlation to magic spot nucleotides
The inositol pyrophosphate metabolism of Dictyostelium discoideum does not regulate inorganic polyphosphate (polyP) synthesis
Make or break: the thermodynamic equilibrium of polyphosphate kinase-catalysed reactions
Vtc5, a novel subunit of the vacuolar transporter chaperone complex, regulates polyphosphate synthesis and phosphate homeostasis in yeast
The 8th young faculty meeting – an active crowd attuned to modern challenges
Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems
Development of novel p-amidites and their application in the synthesis of the most densely phosphorylated compounds
Untersuchungen zur intrazellulären Freisetzung von Nucleosidphosphaten
Synthesis of bisdiphosphoinositol polyphosphate prometabolites
Characterisation of family 2 polyphosphate kinases
An update on polyphosphate in vivo activities
Metabolic consequences of polyphosphate synthesis and imminent phosphate limitation
Investigations on the synthesis and degradation of polyphosphate in vitro and its correlation to magic spot nucleotides
The inositol pyrophosphate metabolism of Dictyostelium discoideum does not regulate inorganic polyphosphate (polyP) synthesis
Make or break: the thermodynamic equilibrium of polyphosphate kinase-catalysed reactions
Vtc5, a novel subunit of the vacuolar transporter chaperone complex, regulates polyphosphate synthesis and phosphate homeostasis in yeast
The 8th young faculty meeting – an active crowd attuned to modern challenges
Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems