Radical Transfer Dissociation for De Novo Characterization of Modified Ribonucleic Acids by Mass Spectrometry

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Abstract: Mass spectrometry (MS) can reliably detect and localize all mass‐altering modifications of ribonucleic acids (RNA), but current MS approaches that allow for simultaneous de novo sequencing and modification analysis generally require specialized instrumentation. Here we report a novel RNA dissociation technique, radical transfer dissociation (RTD), that can be used for the comprehensive de novo characterization of ribonucleic acids and their posttranscriptional or synthetic modifications. We demonstrate full sequence coverage for RNA consisting of up to 39 nucleotides and show that RTD is especially useful for RNA with highly labile modifications such as 5‐hydroxymethylcytidine and 5‐formylcytidine.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Radical Transfer Dissociation for De Novo Characterization of Modified Ribonucleic Acids by Mass Spectrometry ; volume:59 ; number:11 ; year:2020 ; pages:4309-4313 ; extent:5
Angewandte Chemie / International edition. International edition ; 59, Heft 11 (2020), 4309-4313 (gesamt 5)

Creator: Calderisi, Giovanni
Glasner, Heidelinde
Breuker, Kathrin

DOI: 10.1002/anie.201914275

URN: urn:nbn:de:101:1-2022061411323832596208

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 15.08.2025, 7:26 AM CEST

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Associated

Calderisi, Giovanni
Glasner, Heidelinde
Breuker, Kathrin

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Modified—modified radical mastoidectomy

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Determination of dissociation constants via quantitative mass spectrometry

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OH Radical‐Induced Oxidation in Nucleosides and Nucleotides Unraveled by Tandem Mass Spectrometry and Infrared Multiple Photon Dissociation Spectroscopy

OH Radical‐Induced Oxidation in Nucleosides and Nucleotides Unraveled by Tandem Mass Spectrometry and Infrared Multiple Photon Dissociation Spectroscopy

Modified Oesophago-Gastric Dissociation (M-OGD) — a technical modification

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Estimation of bond dissociation energies and radical stabilization energies by ESR spectroscopy

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O2 dissociation in Na-modified gold ultrathin layer on Cu(111)

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Silicon Radical‐Induced CH 4 Dissociation for Uniform Graphene Coating on Silica Surface

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Radical Transfer Dissociation for De Novo Characterization of Modified Ribonucleic Acids by Mass Spectrometry

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Modified—modified radical mastoidectomy

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Determination of dissociation constants via quantitative mass spectrometry

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OH Radical‐Induced Oxidation in Nucleosides and Nucleotides Unraveled by Tandem Mass Spectrometry and Infrared Multiple Photon Dissociation Spectroscopy

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Modified Oesophago-Gastric Dissociation (M-OGD) — a technical modification

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Comparison between Source-induced Dissociation and Collision-induced Dissociation of Ampicillin, Chloramphenicol, Ciprofloxacin, and Oxytetracycline via Mass Spectrometry

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Estimation of bond dissociation energies and radical stabilization energies by ESR spectroscopy

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O2 dissociation in Na-modified gold ultrathin layer on Cu(111)

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Silicon Radical‐Induced CH 4 Dissociation for Uniform Graphene Coating on Silica Surface

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