Radical Transfer Dissociation for De Novo Characterization of Modified Ribonucleic Acids by Mass Spectrometry
Abstract: Mass spectrometry (MS) can reliably detect and localize all mass‐altering modifications of ribonucleic acids (RNA), but current MS approaches that allow for simultaneous de novo sequencing and modification analysis generally require specialized instrumentation. Here we report a novel RNA dissociation technique, radical transfer dissociation (RTD), that can be used for the comprehensive de novo characterization of ribonucleic acids and their posttranscriptional or synthetic modifications. We demonstrate full sequence coverage for RNA consisting of up to 39 nucleotides and show that RTD is especially useful for RNA with highly labile modifications such as 5‐hydroxymethylcytidine and 5‐formylcytidine.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Radical Transfer Dissociation for De Novo Characterization of Modified Ribonucleic Acids by Mass Spectrometry ; volume:59 ; number:11 ; year:2020 ; pages:4309-4313 ; extent:5
Angewandte Chemie / International edition. International edition ; 59, Heft 11 (2020), 4309-4313 (gesamt 5)
- Urheber
- DOI
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10.1002/anie.201914275
- URN
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urn:nbn:de:101:1-2022061411323832596208
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:26 MESZ
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Beteiligte
- Calderisi, Giovanni
- Glasner, Heidelinde
- Breuker, Kathrin
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