High affinity binding of Escherichia coli cytotoxic necrotizing Factor 1 (CNF1) to Lu/BCAM adhesion glycoprotein

zu Verbundenen Objekten

Abstract: The protein toxin Cytotoxic Necrotizing Factor 1 (CNF1) is a major virulence factor of pathogenic Escherichia coli strains. It belongs to a family of single chain AB-toxins, which enter mammalian cells by receptor-mediated endocytosis. Recently, we identified the Lutheran (Lu) adhesion glycoprotein/basal cell adhesion molecule (BCAM) as a cellular receptor for CNF1. Here, we identified the Ig-like domain 2 of Lu/BCAM as main interaction site of the toxin by direct protein-protein interaction and competition studies. Using surface plasmon resonance, we showed a high affinity CNF-Lu/BCAM interaction with a KD of 2.8 nM. Furthermore, we performed small-angle X-ray scattering to define the molecular envelope of the Lu/BCAM-CNF1 complex, suggesting a 6:1 ratio of Lu/BCAM to CNF1 in the receptor-toxin complex. This study leads to a deeper understanding of the interaction between CNF1 and Lu/BCAM, and presents novel opportunities for the development of future anti-toxin strategies

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch
Anmerkungen
Toxins. 10, 1 (2018), article No. 3, DOI 10.3390/toxins10010003, issn: 2072-6651

Klassifikation
Biowissenschaften, Biologie
Schlagwort
Laminin
Toxin
Rezeptor
Sichelzellenanämie

Ereignis
Veröffentlichung
(wo)
Freiburg
(wer)
Universität
(wann)
2018
Urheber
Reppin, Franziska
Cochet, Sylvie
El Nemer, Wassim
Fritz, Günter
Schmidt, Gudula
Beteiligte Personen und Organisationen

DOI
10.3390/toxins10010003
URN
urn:nbn:de:bsz:25-freidok-151247
Rechteinformation
Kein Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
25.03.2025, 13:47 MEZ

Datenpartner

Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Original beim Datenpartner anzeigen

Beteiligte

Entstanden

  • 2018

Ähnliche Objekte (12)

Lu/BCAM Adhesion Glycoprotein Is a Receptor for Escherichia coli Cytotoxic Necrotizing Factor 1 (CNF1)

Lu/BCAM Adhesion Glycoprotein Is a Receptor for Escherichia coli Cytotoxic Necrotizing Factor 1 (CNF1)

Lu/BCAM adhesion glycoprotein is a receptor for Escherichia coli Cytotoxic Necrotizing Factor 1 (CNF1)

Lu/BCAM adhesion glycoprotein is a receptor for Escherichia coli Cytotoxic Necrotizing Factor 1 (CNF1)

Characterization of Galectin Fusion Proteins with Glycoprotein Affinity Columns and Binding Assays

Characterization of Galectin Fusion Proteins with Glycoprotein Affinity Columns and Binding Assays

Affinity proteomics identifies novel functional modules related to adhesion GPCRs

Affinity proteomics identifies novel functional modules related to adhesion GPCRs

Affinity

Buch

Affinity

Affinity

Buch

Affinity

The uptake of the Escherichia coli toxin cytotoxic necrotizing factor 1 into host cells

Hochschulschrift

The uptake of the Escherichia coli toxin cytotoxic necrotizing factor 1 into host cells

Conditionally cytotoxic and drug-controllable non-cytotoxic rabies viruses

Conditionally cytotoxic and drug-controllable non-cytotoxic rabies viruses

Cytotoxic Granule Endocytosis in Primary Mouse Cytotoxic T Lymphocytes

Cytotoxic Granule Endocytosis in Primary Mouse Cytotoxic T Lymphocytes

Cytotoxic effector mechanisms

Aufsatzsammlung

Cytotoxic effector mechanisms

Bicyclic RGD peptides : Novel high-affinity ligands for selective integrin-binding and integrin-mediated cell adhesion

Bicyclic RGD peptides : Novel high-affinity ligands for selective integrin-binding and integrin-mediated cell adhesion

Modifikation von α1-Glycoprotein [Alpha-Glycoprotein] (Orosomucoid) aus menschlichem Serum

Hochschulschrift

Modifikation von α1-Glycoprotein [Alpha-Glycoprotein] (Orosomucoid) aus menschlichem Serum

Verbundene Objekte