Mechanistic Characterisation of Collinodiene Synthase, a Diterpene Synthase from Streptomyces collinus
Abstract: Two homologs of the diterpene synthase CotB2 from Streptomyces collinus (ScCotB2) and Streptomyces iakyrus (SiCotB2) were investigated for their products by in vitro incubations of the recombinant enzymes with geranylgeranyl pyrophosphate, followed by compound isolation and structure elucidation by NMR. ScCotB2 produced the new compound collinodiene, besides the canonical CotB2 product cyclooctat‐9‐en‐7‐ol, dolabella‐3,7,18‐triene and dolabella‐3,7,12‐triene, while SiCotB2 gave mainly cyclooctat‐9‐en‐7‐ol and only traces of dolabella‐3,7,18‐triene. The cyclisation mechanism towards the ScCotB2 products and their absolute configurations were investigated through isotopic labelling experiments.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Mechanistic Characterisation of Collinodiene Synthase, a Diterpene Synthase from Streptomyces collinus ; day:02 ; month:10 ; year:2023 ; extent:7
Chemistry - a European journal ; (02.10.2023) (gesamt 7)
- Creator
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Taizoumbe, Kizerbo A.
Steiner, Simon T.
Dickschat, Jeroen S.
- DOI
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10.1002/chem.202302469
- URN
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urn:nbn:de:101:1-2023100315301406051310
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:47 AM CEST
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Associated
- Taizoumbe, Kizerbo A.
- Steiner, Simon T.
- Dickschat, Jeroen S.
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