Mechanistic Characterisation of the Bacterial Sesterviridene Synthase from Kitasatospora viridis

to related objects

Abstract: A gene coding for a terpene synthase homolog from Kitasatospora viridis was cloned and expressed in Escherichia coli. The purified recombinant protein possessed sesterterpene synthase activity and efficiently converted geranylfarnesyl diphosphate (GFPP) with 19 % yield into the sesterterpene hydrocarbon sesterviridene A. Large scale enzymatic conversions also allowed for the isolation of two side products that are generated with very low yields of ca. 0.1 %. Several derivatives of sesterviridene A were obtained by chemical transformations, securing the NMR‐based structural assignments. The absolute configuration of sesterviridene A was determined by chemical correlation using stereoselectively deuterated precursors and by anomalous dispersion X‐ray crystallography. The cyclisation mechanism from GFPP to sesterviridene A was extensively studied through isotopic labelling experiments and DFT calculations.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Mechanistic Characterisation of the Bacterial Sesterviridene Synthase from Kitasatospora viridis ; day:23 ; month:06 ; year:2023 ; extent:6
Angewandte Chemie / International edition. International edition ; (23.06.2023) (gesamt 6)

Creator: Xu, Houchao
Schnakenburg, Gregor
Goldfuss, Bernd
Dickschat, Jeroen S.

DOI: 10.1002/anie.202306429

URN: urn:nbn:de:101:1-2023062315425709383195

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 14.08.2025, 10:52 AM CEST

Data provider

This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.

Show original at data provider

Associated

Xu, Houchao
Schnakenburg, Gregor
Goldfuss, Bernd
Dickschat, Jeroen S.

Other Objects (12)

Mechanistic Characterisation of Collinodiene Synthase, a Diterpene Synthase from Streptomyces collinus

Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens

Mechanistische Charakterisierung der Bakteriellen Sesterviriden‐Synthase aus Kitasatospora viridis

Mechanistic Analysis and Antibiotic Target Validation of Aminodeoxychorismate Synthase and Anthranilate Synthase

Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica

Hochschulschrift

Mechanistic studies on bacterial terpene synthases

Structural and Biophysical Characterization of the Bacterial Homospermidine Synthase from Blastochloris viridis to Provide Insights into Substrate Recognition and Reaction Mechanism

Fotografie

Rochlitz. Wechselkröte (Bufotes viridis, Synonym: Bufo viridis, gelegentlich Pseudepidalea viridis)

Mechanistic investigations on six bacterial terpene cyclases

A mechanistic understanding of bacterial chromosome organisation

Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase

Mechanistic investigations on multiproduct β-himachalene synthase from Cryptosporangium arvum

Mechanistic Characterisation of Collinodiene Synthase, a Diterpene Synthase from Streptomyces collinus

Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens

Mechanistische Charakterisierung der Bakteriellen Sesterviriden‐Synthase aus Kitasatospora viridis

Mechanistic Analysis and Antibiotic Target Validation of Aminodeoxychorismate Synthase and Anthranilate Synthase

Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica

Hochschulschrift

Mechanistic studies on bacterial terpene synthases

Structural and Biophysical Characterization of the Bacterial Homospermidine Synthase from Blastochloris viridis to Provide Insights into Substrate Recognition and Reaction Mechanism

Fotografie

Rochlitz. Wechselkröte (Bufotes viridis, Synonym: Bufo viridis, gelegentlich Pseudepidalea viridis)

Mechanistic investigations on six bacterial terpene cyclases

A mechanistic understanding of bacterial chromosome organisation

Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase

Mechanistic investigations on multiproduct β-himachalene synthase from Cryptosporangium arvum

Mechanistic Characterisation of Collinodiene Synthase, a Diterpene Synthase from Streptomyces collinus

Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens

Mechanistische Charakterisierung der Bakteriellen Sesterviriden‐Synthase aus Kitasatospora viridis

Mechanistic Analysis and Antibiotic Target Validation of Aminodeoxychorismate Synthase and Anthranilate Synthase

Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica

Hochschulschrift

Mechanistic studies on bacterial terpene synthases

Structural and Biophysical Characterization of the Bacterial Homospermidine Synthase from Blastochloris viridis to Provide Insights into Substrate Recognition and Reaction Mechanism

Fotografie

Rochlitz. Wechselkröte (Bufotes viridis, Synonym: Bufo viridis, gelegentlich Pseudepidalea viridis)

Mechanistic investigations on six bacterial terpene cyclases

A mechanistic understanding of bacterial chromosome organisation

Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase

Mechanistic investigations on multiproduct β-himachalene synthase from Cryptosporangium arvum

Cultural heritage institutions wishing to register will find more information here.

Fields marked * need to be filled in.

Username*

Please enter your username

Email*

Please enter your email address

Please do not fill this field

First name

Last name

Password*

Please enter your password

Confirm password*

Please enter the same password

I have read the terms of use and the privacy policy for the collection of personal data and accept them. *

This field is required.

I would like to subscribe to the newsletter of the Deutsche Digitale Bibliothek. See newsletter subscription info.

Account created

Your "My DDB" account has been successfully created. Before you can log in to your account, you must click the confirmation link in the message we just sent to the email address you provided.

Mechanistic Characterisation of the Bacterial Sesterviridene Synthase from Kitasatospora viridis

Object Details

References and Relationships

Contributors, Places and Time

Further information

Data provider

Associated

Other Objects (12)

Mechanistic Characterisation of Collinodiene Synthase, a Diterpene Synthase from Streptomyces collinus

Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens

Mechanistische Charakterisierung der Bakteriellen Sesterviriden‐Synthase aus Kitasatospora viridis

Mechanistic Analysis and Antibiotic Target Validation of Aminodeoxychorismate Synthase and Anthranilate Synthase

Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica

Mechanistic studies on bacterial terpene synthases

Structural and Biophysical Characterization of the Bacterial Homospermidine Synthase from Blastochloris viridis to Provide Insights into Substrate Recognition and Reaction Mechanism

Rochlitz. Wechselkröte (Bufotes viridis, Synonym: Bufo viridis, gelegentlich Pseudepidalea viridis)

Mechanistic investigations on six bacterial terpene cyclases

A mechanistic understanding of bacterial chromosome organisation

Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase

Mechanistic investigations on multiproduct β-himachalene synthase from Cryptosporangium arvum

Mechanistic Characterisation of Collinodiene Synthase, a Diterpene Synthase from Streptomyces collinus

Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens

Mechanistische Charakterisierung der Bakteriellen Sesterviriden‐Synthase aus Kitasatospora viridis

Mechanistic Analysis and Antibiotic Target Validation of Aminodeoxychorismate Synthase and Anthranilate Synthase

Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica

Mechanistic studies on bacterial terpene synthases

Structural and Biophysical Characterization of the Bacterial Homospermidine Synthase from Blastochloris viridis to Provide Insights into Substrate Recognition and Reaction Mechanism

Rochlitz. Wechselkröte (Bufotes viridis, Synonym: Bufo viridis, gelegentlich Pseudepidalea viridis)

Mechanistic investigations on six bacterial terpene cyclases

A mechanistic understanding of bacterial chromosome organisation

Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase

Mechanistic investigations on multiproduct β-himachalene synthase from Cryptosporangium arvum

Mechanistic Characterisation of Collinodiene Synthase, a Diterpene Synthase from Streptomyces collinus

Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens

Mechanistische Charakterisierung der Bakteriellen Sesterviriden‐Synthase aus Kitasatospora viridis

Mechanistic Analysis and Antibiotic Target Validation of Aminodeoxychorismate Synthase and Anthranilate Synthase

Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica

Mechanistic studies on bacterial terpene synthases

Structural and Biophysical Characterization of the Bacterial Homospermidine Synthase from Blastochloris viridis to Provide Insights into Substrate Recognition and Reaction Mechanism

Rochlitz. Wechselkröte (Bufotes viridis, Synonym: Bufo viridis, gelegentlich Pseudepidalea viridis)

Mechanistic investigations on six bacterial terpene cyclases

A mechanistic understanding of bacterial chromosome organisation

Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase

Mechanistic investigations on multiproduct β-himachalene synthase from Cryptosporangium arvum

Related objects

Reset password