Exometabolom analysis of breast cancer cell lines: metabolic signature

Abstract: Cancer cells show characteristic effects on cellular turnover and DNA/RNA modifications leading to elevated levels of excreted modified nucleosides. We investigated the molecular signature of different subtypes of breast cancer cell lines and the breast epithelial cell line MCF-10A. Prepurification of cell culture supernatants was performed by cis-diol specific affinity chromatography using boronate-derivatized polyacrylamide gel. Samples were analyzed by application of reversed phase chromatography coupled to a triple quadrupole mass spectrometer. Collectively, we determined 23 compounds from RNA metabolism, two from purine metabolism, five from polyamine/methionine cycle, one from histidine metabolism and two from nicotinate and nicotinamide metabolism. We observed major differences of metabolite excretion pattern between the breast cancer cell lines and MCF-10A, just as well as between the different breast cancer cell lines themselves. Differences in metabolite excretion resulting from cancerous metabolism can be integrated into altered processes on the cellular level. Modified nucleosides have great potential as biomarkers in due consideration of the heterogeneity of breast cancer that is reflected by the different molecular subtypes of breast cancer. Our data suggests that the metabolic signature of breast cancer cell lines might be a more subtype-specific tool to predict breast cancer, rather than a universal approach

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch
• Anmerkungen: Scientific reports. 5 (2015), 13374, DOI 10.1038/srep13374, issn: 2045-2322 ; IN COPYRIGHT http://rightsstatements.org/page/InC/1.0 rs

Klassifikation und Themen

• Schlagwort: Krebs ; Onkologie

Beteiligte, Orts- und Zeitangaben

• Ereignis: Veröffentlichung
• (wo): Freiburg
• (wer): Universität
• (wann): 2015
• Urheber: Willmann, Lucas ; Erbes, Thalia ; Halbach, Sebastian ; Brummer, Tilman ; Jäger, Markus ; Stickeler, Elmar ; Kammerer, Bernd
• Beteiligte Personen und Organisationen: Zentrum für Biosystemanalyse ; Klinik für Frauenheilkunde ; Albert-Ludwigs-Universität Freiburg. Institut für Molekulare Medizin und Zellforschung ; Albert-Ludwigs-Universität Freiburg. Institut für Biologie II ; Albert-Ludwigs-Universität Freiburg. Institut für Biologie III ; Spemann Graduate School of Biology and Medicine ; Albert-Ludwigs-Universität Freiburg. Medizinische Fakultät ; Albert-Ludwigs-Universität Freiburg

Weitere Informationen

• DOI: 10.1038/srep13374
• URN: urn:nbn:de:bsz:25-freidok-120803
• Rechteinformation: Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 25.03.2025, 13:46 MEZ