Hydrophobization of a TIP60 Protein Nanocage for the Encapsulation of Hydrophobic Compounds
Abstract: Encapsulation of hydrophobic molecules in protein‐based nanocages is a promising approach for dispersing these molecules in water. Here, we report a chemical modification approach to produce a protein nanocage with a hydrophobic interior surface based on our previously developed nanocage, TIP60. The large pores of TIP60 act as tunnels for small molecules, allowing modification of the interior surface by hydrophobic compounds without nanocage disassembly. We used four different hydrophobic compounds for modification. The largest modification group tested, pyrene, resulted in a modified TIP60 that could encapsulate aromatic photosensitizer zinc phthalocyanine (ZnPC) more efficiently than the other modification compounds. The encapsulated ZnPC generated singlet oxygen upon light activation in the aqueous phase, whereas ZnPC alone formed inert aggregates under the same experimental conditions. Given that chemical modification allows a wider diversity of modifications than mutagenesis, this approach could be used to develop more suitable nanocages for encapsulating hydrophobic molecules of interest.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Hydrophobization of a TIP60 Protein Nanocage for the Encapsulation of Hydrophobic Compounds ; volume:88 ; number:3 ; year:2023 ; extent:6
ChemPlusChem ; 88, Heft 3 (2023) (gesamt 6)
- Creator
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Yamashita, Maika
Kawakami, Norifumi
Miyamoto, Kenji
- DOI
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10.1002/cplu.202200392
- URN
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urn:nbn:de:101:1-2023030114342780047679
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:57 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Yamashita, Maika
- Kawakami, Norifumi
- Miyamoto, Kenji
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