Surveillance and genomic analysis of third-generation cephalosporin-resistant and carbapenem-resistant klebsiella pneumoniae complex in Germany

Abstract: To analyse the epidemiology and population structure of third-generation cephalosporin-resistant (3GCR) and carbapenem-resistant (CR) Klebsiella pneumoniae complex isolates, patients were screened for rectal colonisation with 3GCR/CR K. pneumoniae complex on admission to six German university hospitals (2016–2019). Also collected were 3GCR/CR and susceptible K. pneumoniae isolates from patients with bloodstream infections (2016–2018). Whole-genome sequencing was performed followed by multilocus sequencing typing (MLST), core-genome MLST, and resistome and virulome analysis. The admission prevalence of 3GCR K. pneumoniae complex isolates during the 4-year study period was 0.8%, and 1.0 bloodstream infection per 1000 patient admissions was caused by K. pneumoniae complex (3GCR prevalence, 15.1%). A total of seven K. pneumoniae complex bloodstream isolates were CR (0.8%). The majority of colonising and bloodstream 3GCR isolates were identified as K. pneumoniae, 96.7% and 98.8%, respectively; the remainder were K. variicola and K. quasipneumoniae. cgMLST showed a polyclonal population of colonising and bloodstream isolates, which was also reflected by MLST and virulome analysis. CTX-M-15 was the most prevalent extended-spectrum beta-lactamase, and 29.7% of the colonising and 48.8% of the bloodstream isolates were high-risk clones. The present study provides an insight into the polyclonal 3GCR K. pneumoniae population in German hospitals

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch
• Anmerkungen: Antibiotics. - 11, 10 (2022) , 1286, ISSN: 2079-6382

Beteiligte, Orts- und Zeitangaben

• Ereignis: Veröffentlichung
• (wo): Freiburg
• (wer): Universität
• (wann): 2022
• Urheber: Xanthopoulou, Kyriaki ; Imirzalioglu, Can ; Walker, Sarah V. ; Behnke, Michael ; Dinkelacker, Ariane G. ; Eisenbeis, Simone ; Gastmeier, Petra ; Gölz, Hanna ; Käding, Nadja ; Kern, Winfried V. ; Kola, Axel ; Kramme, Evelyn ; Lucassen, Kai ; Mischnik, Alexander ; Peter, Silke ; Rohde, Anna Maria ; Rupp, Jan ; Tacconelli, Evelina ; Tobys, David ; Vehreschild, Maria J. G. T. ; Wille, Julia ; Seifert, Harald ; Higgins, Paul G. ; Häcker, Georg ; Peyerl-Hoffmann, Gabriele ; Schuster, Sabine ; Vavra, Martina

Weitere Informationen

• DOI: 10.3390/antibiotics11101286
• URN: urn:nbn:de:bsz:25-freidok-2304537
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 25.03.2025, 13:46 MEZ