Carboxyl Methyltransferase Catalysed Formation of Mono‐ and Dimethyl Esters under Aqueous Conditions: Application in Cascade Biocatalysis
Abstract: Carboxyl methyltransferase (CMT) enzymes catalyse the biomethylation of carboxylic acids under aqueous conditions and have potential for use in synthetic enzyme cascades. Herein we report that the enzyme FtpM from Aspergillus fumigatus can methylate a broad range of aromatic mono‐ and dicarboxylic acids in good to excellent conversions. The enzyme shows high regioselectivity on its natural substrate fumaryl‐l‐tyrosine, trans, trans‐muconic acid and a number of the dicarboxylic acids tested. Dicarboxylic acids are generally better substrates than monocarboxylic acids, although some substituents are able to compensate for the absence of a second acid group. For dicarboxylic acids, the second methylation shows strong pH dependency with an optimum at pH 5.5–6. Potential for application in industrial biotechnology was demonstrated in a cascade for the production of a bioplastics precursor (FDME) from bioderived 5‐hydroxymethylfurfural (HMF).
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Carboxyl Methyltransferase Catalysed Formation of Mono‐ and Dimethyl Esters under Aqueous Conditions: Application in Cascade Biocatalysis ; day:16 ; month:02 ; year:2022 ; extent:1
Angewandte Chemie / International edition. International edition ; (16.02.2022) (gesamt 1)
- Creator
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Ward, Lucy C.
McCue, Hannah V.
Rigden, Daniel J.
Kershaw, Neil M.
Ashbrook, Chloe
Hatton, Harry
Goulding, Ellie
Johnson, James R.
Carnell, Andrew J.
- DOI
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10.1002/anie.202117324
- URN
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urn:nbn:de:101:1-2022021714044131533797
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:30 AM CEST
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Associated
- Ward, Lucy C.
- McCue, Hannah V.
- Rigden, Daniel J.
- Kershaw, Neil M.
- Ashbrook, Chloe
- Hatton, Harry
- Goulding, Ellie
- Johnson, James R.
- Carnell, Andrew J.
Other Objects (12)
Carboxyl Methyltransferase Catalysed Formation of Mono‐ and Dimethyl Esters under Aqueous Conditions: Application in Cascade Biocatalysis
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Engineering a Carboxyl Methyltransferase for the Formation of a Furan‐Based Bioplastic Precursor
Eine Struktur- und Funktionsanalyse der floralen Salizylsäure-Carboxyl-Methyltransferase aus Hoya carnosa R. Br.
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Base-catalysed cleavage of lignin β-O-4 model compounds in dimethyl carbonate
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Enzymatic synthesis of l‐methionine analogues and application in a methyltransferase catalysed alkylation cascade
Enzymatic Synthesis of l ‐Methionine Analogues and Application in a Methyltransferase Catalysed Alkylation Cascade **
Cluster Preface: Biocatalysis
Einige Versuche mit Carboxyl-C14-Biotin
Carboxyl Methyltransferase Catalysed Formation of Mono‐ and Dimethyl Esters under Aqueous Conditions: Application in Cascade Biocatalysis
Isoprenyl carboxyl methyltransferase inhibitors: a brief review including recent patents
Hydrosilylation of carbonyl and carboxyl groups catalysed by Mn(i) complexes bearing triazole ligands
Engineering a Carboxyl Methyltransferase for the Formation of a Furan‐Based Bioplastic Precursor
Eine Struktur- und Funktionsanalyse der floralen Salizylsäure-Carboxyl-Methyltransferase aus Hoya carnosa R. Br.
Eine Struktur- und Funktionsanalyse der floralen Salizylsäure-Carboxyl-Methyltransferase aus Hoya carnosa R. Br.
Base-catalysed cleavage of lignin β-O-4 model compounds in dimethyl carbonate
Cascading Biocatalysis
Enzymatic synthesis of l‐methionine analogues and application in a methyltransferase catalysed alkylation cascade
Enzymatic Synthesis of l ‐Methionine Analogues and Application in a Methyltransferase Catalysed Alkylation Cascade **
Cluster Preface: Biocatalysis
Einige Versuche mit Carboxyl-C14-Biotin
Carboxyl Methyltransferase Catalysed Formation of Mono‐ and Dimethyl Esters under Aqueous Conditions: Application in Cascade Biocatalysis
Isoprenyl carboxyl methyltransferase inhibitors: a brief review including recent patents
Hydrosilylation of carbonyl and carboxyl groups catalysed by Mn(i) complexes bearing triazole ligands
Engineering a Carboxyl Methyltransferase for the Formation of a Furan‐Based Bioplastic Precursor
Eine Struktur- und Funktionsanalyse der floralen Salizylsäure-Carboxyl-Methyltransferase aus Hoya carnosa R. Br.
Eine Struktur- und Funktionsanalyse der floralen Salizylsäure-Carboxyl-Methyltransferase aus Hoya carnosa R. Br.
Base-catalysed cleavage of lignin β-O-4 model compounds in dimethyl carbonate
Cascading Biocatalysis
Enzymatic synthesis of l‐methionine analogues and application in a methyltransferase catalysed alkylation cascade
Enzymatic Synthesis of l ‐Methionine Analogues and Application in a Methyltransferase Catalysed Alkylation Cascade **
Cluster Preface: Biocatalysis