Light-sheet microscopy with length-adaptive Bessel beams
Abstract: In light-sheet microscopy, a confined layer in the focal plane of the detection objective is illuminated from the side. The illumination light-sheet usually has a constant beam length independent of the shape of the biological object. Since the thickness and the length of the illumination light-sheet are coupled, a tradeoff between resolution, contrast and field of view has to be accepted. Here we show that scanned Bessel beams enable object adapted tailoring of the light-sheet defined by its beam length and position. The individual beam parameters are obtained from automatic object shape estimation by low-power laser light scattered at the object. Using Arabidopsis root tips, cell clusters and zebrafish tails, we demonstrate that Bessel beam light-sheet tailoring leads to a 50% increase in image contrast and a 50% reduction in photobleaching. Light-sheet tailoring requires only binary amplitude modulation, therefore allowing a real time illumination adaptation with little technical effort in the future
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Anmerkungen
-
issn: 2156-7085
- Schlagwort
-
Lichtscheibenmikroskopie
Bessel-Bündel
- Ereignis
-
Veröffentlichung
- (wo)
-
Freiburg
- (wer)
-
Universität
- (wann)
-
2019
- Urheber
- DOI
-
10.1364/BOE.10.000670
- URN
-
urn:nbn:de:bsz:25-freidok-1492692
- Rechteinformation
-
Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
14.08.2025, 10:46 MESZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Meinert, Tobias
- Rohrbach, Alexander
- Universität
Entstanden
- 2019
Ähnliche Objekte (12)
A shape-switch-block method for confocal light-sheet microscopy with sectioned Bessel beams and stimulated emission depletion
A shape-switch-block method for confocal light-sheet microscopy with sectioned Bessel beams and stimulated emission depletion
Microscopy with self-reconstructing beams : = Mikroskopie mit selbstrekonstruierenden Strahlen
Making Hidden Cell Particle Interactions Visible by Thermal Noise Frequency Decomposition
Total internal reflection microscopy: super-resolution imaging of bacterial dynamics and dark field imaging : = Totalreflexionsmikroskopie: Superauflösende Mikroskopie von Dynamik in Bakterien und Dunkelfeld Mikroskopie
ROCS microscopy with distinct zero-order blocking
Investigation of filopodia dynamics in macrophage cells by photonic force microscopy
Super-resolution structured illumination microscopy of MreB dynamics and cell wall synthesis in B. subtilis
Sortieren und Manipulieren von biologischen Objekten mit optischen Fallen und interferometrischem Tracking
Interferometrisches Tracking und Manipulation von Nanorods mit optischen Pinzetten
Surface scanning using optically trapped probes : = Oberflächenuntersuchungen mit optisch gefangenen Sonden
Biomechanics of prokaryotic & eukaryotic cytoskeletal model systems probed by time-multiplexed optical tweezers
A shape-switch-block method for confocal light-sheet microscopy with sectioned Bessel beams and stimulated emission depletion
A shape-switch-block method for confocal light-sheet microscopy with sectioned Bessel beams and stimulated emission depletion
Microscopy with self-reconstructing beams : = Mikroskopie mit selbstrekonstruierenden Strahlen
Making Hidden Cell Particle Interactions Visible by Thermal Noise Frequency Decomposition
Total internal reflection microscopy: super-resolution imaging of bacterial dynamics and dark field imaging : = Totalreflexionsmikroskopie: Superauflösende Mikroskopie von Dynamik in Bakterien und Dunkelfeld Mikroskopie
ROCS microscopy with distinct zero-order blocking
Investigation of filopodia dynamics in macrophage cells by photonic force microscopy
Super-resolution structured illumination microscopy of MreB dynamics and cell wall synthesis in B. subtilis
Sortieren und Manipulieren von biologischen Objekten mit optischen Fallen und interferometrischem Tracking
Interferometrisches Tracking und Manipulation von Nanorods mit optischen Pinzetten
Surface scanning using optically trapped probes : = Oberflächenuntersuchungen mit optisch gefangenen Sonden
Biomechanics of prokaryotic & eukaryotic cytoskeletal model systems probed by time-multiplexed optical tweezers
A shape-switch-block method for confocal light-sheet microscopy with sectioned Bessel beams and stimulated emission depletion
A shape-switch-block method for confocal light-sheet microscopy with sectioned Bessel beams and stimulated emission depletion
Microscopy with self-reconstructing beams : = Mikroskopie mit selbstrekonstruierenden Strahlen
Making Hidden Cell Particle Interactions Visible by Thermal Noise Frequency Decomposition
Total internal reflection microscopy: super-resolution imaging of bacterial dynamics and dark field imaging : = Totalreflexionsmikroskopie: Superauflösende Mikroskopie von Dynamik in Bakterien und Dunkelfeld Mikroskopie
ROCS microscopy with distinct zero-order blocking
Investigation of filopodia dynamics in macrophage cells by photonic force microscopy
Super-resolution structured illumination microscopy of MreB dynamics and cell wall synthesis in B. subtilis
Sortieren und Manipulieren von biologischen Objekten mit optischen Fallen und interferometrischem Tracking
Interferometrisches Tracking und Manipulation von Nanorods mit optischen Pinzetten
Surface scanning using optically trapped probes : = Oberflächenuntersuchungen mit optisch gefangenen Sonden