Protein glutaminylation is a yeast-specific posttranslational modification of elongation factor 1A

Abstract: Ribosomal translation factors are fundamental for protein synthesis and highly conserved in all kingdoms of life. The essential eukaryotic elongation factor 1A (eEF1A) delivers aminoacyl tRNAs to the A-site of the translating 80S ribosome. Several studies have revealed that eEF1A is posttranslationally modified. Using MS analysis, site-directed mutagenesis, and X-ray structural data analysis of Saccharomyces cerevisiae eEF1A, we identified a posttranslational modification in which the α amino group of mono-l-glutamine is covalently linked to the side chain of glutamate 45 in eEF1A. The MS analysis suggested that all eEF1A molecules are modified by this glutaminylation and that this posttranslational modification occurs at all stages of yeast growth. The mutational studies revealed that this glutaminylation is not essential for the normal functions of eEF1A in S. cerevisiae. However, eEF1A glutaminylation slightly reduced growth under antibiotic-induced translational stress conditions. Moreover, we identified the same posttranslational modification in eEF1A from Schizosaccharomyces pombe but not in various other eukaryotic organisms tested despite strict conservation of the Glu45 residue among these organisms. We therefore conclude that eEF1A glutaminylation is a yeast-specific posttranslational modification that appears to influence protein translation

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch
• Anmerkungen: The journal of biological chemistry. - 292, 39 (2017) , 16014-16023, ISSN: 1083-351X

Beteiligte, Orts- und Zeitangaben

• Ereignis: Veröffentlichung
• (wo): Freiburg
• (wer): Universität
• (wann): 2019
• Urheber: Jank, Thomas ; Belyi, Yuri F. ; Wirth, Christophe ; Rospert, Sabine ; Hu, Zehan ; Dengjel, Jörn ; Tzivelekidis, Tina ; Andersen, Gregers Rom ; Hunte, Carola ; Schlosser, Andreas ; Aktories, Klaus
• Beteiligte Personen und Organisationen: Zentrum für Biochemie und Molekulare Zellforschung ; Albert-Ludwigs-Universität Freiburg. Abteilung Pharmakologie I ; FRIAS Natur- und Lebenswissenschaften, Medizin und Ingenieurwissenschaften

Weitere Informationen

• DOI: 10.1074/jbc.m117.801035
• URN: urn:nbn:de:bsz:25-freidok-1408563
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 15.08.2025, 07:25 MESZ