Proteomics characterization of mouse kidney peroxisomes by tandem mass spectrometry and protein correlation profiling

Abstract: The peroxisome represents a ubiquitous single membrane-bound key organelle that executes various metabolic pathways such as fatty acid degradation by α- and β-oxidation, ether-phospholipid biosynthesis, metabolism of reactive oxygen species, and detoxification of glyoxylate in mammals. To fulfil this vast array of metabolic functions, peroxisomes accommodate ∼50 different enzymes at least as identified until now. Interest in peroxisomes has been fueled by the discovery of a group of genetic diseases in humans, which are caused by either a defect in peroxisome biogenesis or the deficient activity of a distinct peroxisomal enzyme or transporter. Although this research has greatly improved our understanding of peroxisomes and their role in mammalian metabolism, deeper insight into biochemistry and functions of peroxisomes is required to expand our knowledge of this low abundance but vital organelle. In this work, we used classical subcellular fractionation in combination with MS-based proteomics methodologies to characterize the proteome of mouse kidney peroxisomes. We could identify virtually all known components involved in peroxisomal metabolism and biogenesis. Moreover through protein localization studies by using a quantitative MS screen combined with statistical analyses, we identified 15 new peroxisomal candidates. Of these, we further investigated five candidates by immunocytochemistry, which confirmed their localization in peroxisomes. As a result of this joint effort, we believe to have compiled the so far most comprehensive protein catalogue of mammalian peroxisomes

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch
Anmerkungen
ISSN: 1535-9476

Ereignis
Veröffentlichung
(wo)
Freiburg
(wer)
Universität
(wann)
2024
Urheber
Wiese, Sebastian Edgar
Gronemeyer, Thomas
Ofman, Rob
Kunze, Markus
Grou, Cláudia P.
Almeida, José A.
Eisenacher, Martin
Stephan, Christian
Hayen, Heiko
Schollenberger, Lukas
Korosec, Thomas
Waterham, Hans R.
Schliebs, Wolfgang
Erdmann, Ralf
Berger, Johannes
Meyer, Helmut E.
Just, Wilhelm W.
Azevedo, Jorge E.
Wanders, Ronald J. A.
Warscheid, Bettina
Grou, Cláudia P.

DOI
10.1074/mcp.m700169-mcp200
URN
urn:nbn:de:bsz:25-freidok-2442028
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
25.03.2025, 13:50 MEZ

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  • 2024

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