Mycobacterium tuberculosis secreted protein ESAT-6 interacts with the human protein syntenin-1

Abstract: In order to study the function of the Mycobacterium tuberculosis protein ESAT-6 in the infection process, we searched for host proteins that interact with this secreted mycobacterial protein. Using a yeast two-hybrid system we identified the rat syntenin-1 protein as a candidate to interact with ESAT-6. This interaction was confirmed in vitro by protein overlay and by surface plasmon resonance using recombinant ESAT-6 and human syntenin-1, and by co-purification analysis of the mycobacterial expressed ESAT-6 and macrophage derived syntenin-1. The interaction domains were localized by two-hybrid studies using truncated derivatives of both proteins and by peptide spot analysis. Two domains of each protein mediate the ESAT-6/syntenin-1 interaction. The C-terminus of ESAT-6 binds to the PDZ-domains of syntenin-1 and the N-terminus of ESAT-6 binds to the N-terminus of syntenin-1. Thus, the host protein syntenin-1 represents a possible cellular receptor for the mycobacterial protein ESAT-6.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Mycobacterium tuberculosis secreted protein ESAT-6 interacts with the human protein syntenin-1 ; volume:1 ; number:2 ; year:2006 ; pages:183-202 ; extent:20 ; Open life sciences ; 1, Heft 2 (2006), 183-202 (gesamt 20)

Contributors, Places and Time

• Creator: Schumann, Gisbert ; Schleier, Susanne ; Rosenkrands, Ida ; Nehmann, Nina ; Hälbich, Steffi ; Zipfel, Peter ; Jonge, Marien ; Cole, Stewart T. ; Munder, Thomas ; Möllmann, Ute

Further information

• DOI: 10.2478/s11535-006-0018-2
• URN: urn:nbn:de:101:1-2409201743458.701555036814
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:38 AM CEST