Mycobacterium tuberculosis secreted protein ESAT-6 interacts with the human protein syntenin-1
Abstract: In order to study the function of the Mycobacterium tuberculosis protein ESAT-6 in the infection process, we searched for host proteins that interact with this secreted mycobacterial protein. Using a yeast two-hybrid system we identified the rat syntenin-1 protein as a candidate to interact with ESAT-6. This interaction was confirmed in vitro by protein overlay and by surface plasmon resonance using recombinant ESAT-6 and human syntenin-1, and by co-purification analysis of the mycobacterial expressed ESAT-6 and macrophage derived syntenin-1. The interaction domains were localized by two-hybrid studies using truncated derivatives of both proteins and by peptide spot analysis. Two domains of each protein mediate the ESAT-6/syntenin-1 interaction. The C-terminus of ESAT-6 binds to the PDZ-domains of syntenin-1 and the N-terminus of ESAT-6 binds to the N-terminus of syntenin-1. Thus, the host protein syntenin-1 represents a possible cellular receptor for the mycobacterial protein ESAT-6.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Mycobacterium tuberculosis secreted protein ESAT-6 interacts with the human protein syntenin-1 ; volume:1 ; number:2 ; year:2006 ; pages:183-202 ; extent:20
Open life sciences ; 1, Heft 2 (2006), 183-202 (gesamt 20)
- Urheber
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Schumann, Gisbert
Schleier, Susanne
Rosenkrands, Ida
Nehmann, Nina
Hälbich, Steffi
Zipfel, Peter
Jonge, Marien
Cole, Stewart T.
Munder, Thomas
Möllmann, Ute
- DOI
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10.2478/s11535-006-0018-2
- URN
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urn:nbn:de:101:1-2409201743458.701555036814
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:38 MESZ
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Beteiligte
- Schumann, Gisbert
- Schleier, Susanne
- Rosenkrands, Ida
- Nehmann, Nina
- Hälbich, Steffi
- Zipfel, Peter
- Jonge, Marien
- Cole, Stewart T.
- Munder, Thomas
- Möllmann, Ute
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