Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells

Abstract: A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500–630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye–ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure–property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one‐ and two‐color images of living cells with an optical resolution of 40–60 nm.

Location
Deutsche Nationalbibliothek Frankfurt am Main
Extent
Online-Ressource
Language
Englisch

Bibliographic citation
Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells ; volume:55 ; number:10 ; year:2016 ; pages:3290-3294 ; extent:5
Angewandte Chemie / International edition. International edition ; 55, Heft 10 (2016), 3290-3294 (gesamt 5)

Creator
Butkevich, Alexey N.
Mitronova, Gyuzel Yu
Sidenstein, Sven C.
Klocke, Jessica L.
Kamin, Dirk
Meineke, Dirk N. H.
D'Este, Elisa
Kraemer, Philip‐Tobias
Danzl, Johann G.
Belov, Vladimir N.
Hell, Stefan W.

DOI
10.1002/anie.201511018
URN
urn:nbn:de:101:1-2022110107414649170226
Rights
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Last update
15.08.2025, 7:26 AM CEST

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Associated

  • Butkevich, Alexey N.
  • Mitronova, Gyuzel Yu
  • Sidenstein, Sven C.
  • Klocke, Jessica L.
  • Kamin, Dirk
  • Meineke, Dirk N. H.
  • D'Este, Elisa
  • Kraemer, Philip‐Tobias
  • Danzl, Johann G.
  • Belov, Vladimir N.
  • Hell, Stefan W.

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