Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells

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Abstract: A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500–630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye–ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure–property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one‐ and two‐color images of living cells with an optical resolution of 40–60 nm.

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch

Erschienen in
Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells ; volume:55 ; number:10 ; year:2016 ; pages:3290-3294 ; extent:5
Angewandte Chemie / International edition. International edition ; 55, Heft 10 (2016), 3290-3294 (gesamt 5)

Urheber
Butkevich, Alexey N.
Mitronova, Gyuzel Yu
Sidenstein, Sven C.
Klocke, Jessica L.
Kamin, Dirk
Meineke, Dirk N. H.
D'Este, Elisa
Kraemer, Philip‐Tobias
Danzl, Johann G.
Belov, Vladimir N.
Hell, Stefan W.

DOI
10.1002/anie.201511018
URN
urn:nbn:de:101:1-2022110107414649170226
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
15.08.2025, 07:26 MESZ

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Beteiligte

  • Butkevich, Alexey N.
  • Mitronova, Gyuzel Yu
  • Sidenstein, Sven C.
  • Klocke, Jessica L.
  • Kamin, Dirk
  • Meineke, Dirk N. H.
  • D'Este, Elisa
  • Kraemer, Philip‐Tobias
  • Danzl, Johann G.
  • Belov, Vladimir N.
  • Hell, Stefan W.

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