Sequenced Somatic Cell Reprogramming and Differentiation Inside Nested Hydrogel Droplets
Abstract: The efficient genesis of pluripotent cells or therapeutic cells for regenerative medicine involves several external manipulations and conditioning protocols, which drives down clinical applicability. Automated programming of the genesis by microscale physical forces and chronological biochemistry can increase clinical success. The design and fabrication of nested polysaccharide droplets (millimeter‐sized) with cell sustaining properties of natural tissues and intrinsic properties for time and space evolution of cell transformation signals between somatic cells, pluripotent cells and differentiated therapeutic cells in a swift and efficient manner without the need for laborious external manipulation are reported. Cells transform between phenotypic states by having single and double nested droplets constituted with extracellular matrix proteins and reprogramming, and differentiation factors infused chronologically across the droplet space. The cell transformation into germ layer cells and bone cells is successfully tested in vitro and in vivo and promotes the formation of new bone tissues. Thus, nested droplets with BMP‐2 loaded guests synthesize mineralized bone tissue plates along the length of a cranial non‐union bone defect at 4 weeks. The advantages of sequenced somatic cell reprogramming and differentiation inside an individual hydrogel module without external manipulation, promoted by formulating tissue mimetic physical, mechanical, and chemical microenvironments are shown.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Sequenced Somatic Cell Reprogramming and Differentiation Inside Nested Hydrogel Droplets ; volume:4 ; number:8 ; year:2020 ; extent:21
Advanced biosystems ; 4, Heft 8 (2020) (gesamt 21)
- Urheber
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Green, David W.
Watson, Jolanta A.
Watson, Gregory S.
Stamboulis, Artemis
- DOI
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10.1002/adbi.202000071
- URN
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urn:nbn:de:101:1-2022061905542406965229
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:26 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Green, David W.
- Watson, Jolanta A.
- Watson, Gregory S.
- Stamboulis, Artemis
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