Sequenced Somatic Cell Reprogramming and Differentiation Inside Nested Hydrogel Droplets
Abstract: The efficient genesis of pluripotent cells or therapeutic cells for regenerative medicine involves several external manipulations and conditioning protocols, which drives down clinical applicability. Automated programming of the genesis by microscale physical forces and chronological biochemistry can increase clinical success. The design and fabrication of nested polysaccharide droplets (millimeter‐sized) with cell sustaining properties of natural tissues and intrinsic properties for time and space evolution of cell transformation signals between somatic cells, pluripotent cells and differentiated therapeutic cells in a swift and efficient manner without the need for laborious external manipulation are reported. Cells transform between phenotypic states by having single and double nested droplets constituted with extracellular matrix proteins and reprogramming, and differentiation factors infused chronologically across the droplet space. The cell transformation into germ layer cells and bone cells is successfully tested in vitro and in vivo and promotes the formation of new bone tissues. Thus, nested droplets with BMP‐2 loaded guests synthesize mineralized bone tissue plates along the length of a cranial non‐union bone defect at 4 weeks. The advantages of sequenced somatic cell reprogramming and differentiation inside an individual hydrogel module without external manipulation, promoted by formulating tissue mimetic physical, mechanical, and chemical microenvironments are shown.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Sequenced Somatic Cell Reprogramming and Differentiation Inside Nested Hydrogel Droplets ; volume:4 ; number:8 ; year:2020 ; extent:21
Advanced biosystems ; 4, Heft 8 (2020) (gesamt 21)
- Creator
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Green, David W.
Watson, Jolanta A.
Watson, Gregory S.
Stamboulis, Artemis
- DOI
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10.1002/adbi.202000071
- URN
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urn:nbn:de:101:1-2022061905542406965229
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:26 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Green, David W.
- Watson, Jolanta A.
- Watson, Gregory S.
- Stamboulis, Artemis
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Conserved regulation of RNA processing in somatic cell reprogramming
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A Stochastic Model of Epigenetic Dynamics in Somatic Cell Reprogramming
Chromosomal aberration arises during somatic reprogramming to pluripotent stem cells
Transcriptional defects and reprogramming barriers in somatic cell nuclear reprogramming as revealed by single-embryo RNA sequencing
Extensive epigenetic reprogramming in human somatic tissues between fetus and adult
Sirt6 regulates efficiency of mouse somatic reprogramming and maintenance of pluripotency
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Metabolic reprogramming and lipid droplets are involved in Zika virus replication in neural cells
Direct reprogramming of somatic cells: an update
Identification of somatic mutations in cancer through Bayesian-based analysis of sequenced genome pairs
Conserved regulation of RNA processing in somatic cell reprogramming
Molecular mechanisms of mouse oocyte : mediated somatic cell nuclear reprogramming
A Stochastic Model of Epigenetic Dynamics in Somatic Cell Reprogramming
Chromosomal aberration arises during somatic reprogramming to pluripotent stem cells
Transcriptional defects and reprogramming barriers in somatic cell nuclear reprogramming as revealed by single-embryo RNA sequencing
Extensive epigenetic reprogramming in human somatic tissues between fetus and adult
Sirt6 regulates efficiency of mouse somatic reprogramming and maintenance of pluripotency
Transcriptional reprogramming of gene expression in bovine somatic cell chromatin transfer embryos
c-MYC Triggers Lipid Remodelling During Early Somatic Cell Reprogramming to Pluripotency