A Bifunctional Spin Label for Ligand Recognition on Surfaces
Abstract: In situ monitoring of biomolecular recognition, especially at surfaces, still presents a significant technical challenge. Electron paramagnetic resonance (EPR) of biomolecules spin‐labeled with nitroxides can offer uniquely sensitive and selective insights into these processes, but new spin‐labeling strategies are needed. The synthesis and study of a bromoacrylaldehyde spin label (BASL), which features two attachment points with orthogonal reactivity is reported. The first examples of mannose and biotin ligands coupled to aqueous carboxy‐functionalized gold nanoparticles through a spin label are presented. EPR spectra were obtained for the spin‐labeled ligands both free in solution and attached to nanoparticles. The labels were recognized by the mannose‐binding lectin, Con A, and the biotin‐binding protein avidin‐peroxidase. Binding gave quantifiable changes in the EPR spectra from which binding profiles could be obtained that reflect the strength of binding in each case.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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A Bifunctional Spin Label for Ligand Recognition on Surfaces ; volume:56 ; number:32 ; year:2017 ; pages:9449-9453 ; extent:5
Angewandte Chemie / International edition. International edition ; 56, Heft 32 (2017), 9449-9453 (gesamt 5)
- Creator
- DOI
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10.1002/anie.201703929
- URN
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urn:nbn:de:101:1-2022091707494949083551
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:31 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Hollas, Michael A.
- Webb, Simon J.
- Flitsch, Sabine
- Fielding, Alistair J.
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