A Bifunctional Spin Label for Ligand Recognition on Surfaces

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Abstract: In situ monitoring of biomolecular recognition, especially at surfaces, still presents a significant technical challenge. Electron paramagnetic resonance (EPR) of biomolecules spin‐labeled with nitroxides can offer uniquely sensitive and selective insights into these processes, but new spin‐labeling strategies are needed. The synthesis and study of a bromoacrylaldehyde spin label (BASL), which features two attachment points with orthogonal reactivity is reported. The first examples of mannose and biotin ligands coupled to aqueous carboxy‐functionalized gold nanoparticles through a spin label are presented. EPR spectra were obtained for the spin‐labeled ligands both free in solution and attached to nanoparticles. The labels were recognized by the mannose‐binding lectin, Con A, and the biotin‐binding protein avidin‐peroxidase. Binding gave quantifiable changes in the EPR spectra from which binding profiles could be obtained that reflect the strength of binding in each case.

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch

Erschienen in
A Bifunctional Spin Label for Ligand Recognition on Surfaces ; volume:56 ; number:32 ; year:2017 ; pages:9449-9453 ; extent:5
Angewandte Chemie / International edition. International edition ; 56, Heft 32 (2017), 9449-9453 (gesamt 5)

Urheber
Hollas, Michael A.
Webb, Simon J.
Flitsch, Sabine
Fielding, Alistair J.

DOI
10.1002/anie.201703929
URN
urn:nbn:de:101:1-2022091707494949083551
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
15.08.2025, 07:31 MESZ

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