Photoaffinity capture compounds to profile the magic spot nucleotide interactomes**
Abstract: Magic Spot Nucleotides (MSN) regulate the stringent response, a highly conserved bacterial stress adaptation mechanism, enabling survival under adverse external challenges. In times of antibiotic crisis, a detailed understanding of stringent response is essential, as potentially new targets for pharmacological intervention could be identified. In this study, we delineate the MSN interactome in Escherichia coli and Salmonella typhimurium applying a family of trifunctional photoaffinity capture compounds. We introduce MSN probes covering a diverse phosphorylation pattern, such as pppGpp, ppGpp, and pGpp. Our chemical proteomics approach provides datasets of putative MSN receptors both from cytosolic and membrane fractions that unveil new MSN targets. We find that the activity of the non‐Nudix hydrolase ApaH is potently inhibited by pppGpp, which itself is converted to pGpp by ApaH. The capture compounds described herein will be useful to identify MSN interactomes across bacterial species
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Angewandte Chemie. International edition. - 61, 22 (2022) , e202201731, ISSN: 1521-3773
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
-
Universität
- (when)
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2022
- DOI
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10.1002/anie.202201731
- URN
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urn:nbn:de:bsz:25-freidok-2267709
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
25.03.2025, 1:55 PM CET
Data provider
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Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
Time of origin
- 2022
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Investigations on the synthesis and degradation of polyphosphate in vitro and its correlation to magic spot nucleotides
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Four phosphates at one blow: access to pentaphosphorylated magic spot nucleotides and their analysis by capillary electrophoresis
The 8th young faculty meeting – an active crowd attuned to modern challenges
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Development of novel p-amidites and their application in the synthesis of the most densely phosphorylated compounds
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Untersuchungen zur intrazellulären Freisetzung von Nucleosidphosphaten
Investigations on the synthesis and degradation of polyphosphate in vitro and its correlation to magic spot nucleotides
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