A 1-phytase type III effector interferes with plant hormone signaling
Abstract: Most Gram-negative phytopathogenic bacteria inject type III effector (T3E) proteins into plant cells to manipulate signaling pathways to the pathogen’s benefit. In resistant plants, specialized immune receptors recognize single T3Es or their biochemical activities, thus halting pathogen ingress. However, molecular function and mode of recognition for most T3Es remains elusive. Here, we show that the Xanthomonas T3E XopH possesses phytase activity, i.e., dephosphorylates phytate (myo-inositol-hexakisphosphate, InsP6), the major phosphate storage compound in plants, which is also involved in pathogen defense. A combination of biochemical approaches, including a new NMR-based method to discriminate inositol polyphosphate enantiomers, identifies XopH as a naturally occurring 1-phytase that dephosphorylates InsP6 at C1. Infection of Nicotiana benthamiana and pepper by Xanthomonas results in a XopH-dependent conversion of InsP6 to InsP5. 1-phytase activity is required for XopH-mediated immunity of plants carrying the Bs7 resistance gene, and for induction of jasmonate- and ethylene-responsive genes in N. benthamiana
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Klassifikation
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Biowissenschaften, Biologie
- Schlagwort
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Phytopathologie
Hydrolasen
NMR-Spektroskopie
Kleines Molekül
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2018
- Urheber
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Blüher, Doreen
- Beteiligte Personen und Organisationen
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Laha, Debabrata
Thieme, Sabine
Hofer, Alexandre
Eschen-Lippold, Lennart
Masch, Antonia
Balcke, Gerd Ulrich
Pavlovic, Igor
Nagel, Oliver
Schonsky, Antje
Hinkelmann, Rahel
Wörner, Jakob
Parvin, Nargis
Greiner, Ralf
Weber, Stefan
Tissier, Alain
Schutkowski, Mike
Lee, Justin Eng Eng
Jessen, Henning Jacob
Schaaf, Gabriel
Bonas, Ulla
- DOI
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10.1038/s41467-017-02195-8
- URN
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urn:nbn:de:bsz:25-freidok-165066
- Rechteinformation
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Kein Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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25.03.2025, 13:52 MEZ
Datenpartner
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Beteiligte
- Blüher, Doreen
- Laha, Debabrata
- Thieme, Sabine
- Hofer, Alexandre
- Eschen-Lippold, Lennart
- Masch, Antonia
- Balcke, Gerd Ulrich
- Pavlovic, Igor
- Nagel, Oliver
- Schonsky, Antje
- Hinkelmann, Rahel
- Wörner, Jakob
- Parvin, Nargis
- Greiner, Ralf
- Weber, Stefan
- Tissier, Alain
- Schutkowski, Mike
- Lee, Justin Eng Eng
- Jessen, Henning Jacob
- Schaaf, Gabriel
- Bonas, Ulla
- Universität
Entstanden
- 2018