Controlled Protein‐Membrane Interactions Modulate Self‐Organization of Min Protein Patterns
Abstract: Self‐organizing protein patterns are crucial for living systems, governing important cellular processes such as polarization and division. While the field of protein self‐organization has reached a point where basic pattern‐forming mechanisms can be reconstituted in vitro using purified proteins, understanding how cells can dynamically switch and modulate these patterns, especially when transiently needed, remains an interesting frontier. Here, we demonstrate the efficient regulation of self‐organizing protein patterns through the modulation of simple biophysical membrane parameters. Our investigation focuses on the impact of membrane affinity changes on Min protein patterns at lipid membranes composed of Escherichia coli lipids or minimal lipid compositions, and we present three major results. First, we observed the emergence of a diverse array of pattern phenotypes, ranging from waves over flower‐shaped patterns to snowflake‐like structures. Second, we demonstrated the dependency of these patterns on the density of protein‐membrane linkers. Finally, we demonstrate that the shape of snowflake‐like patterns is fine‐tuned by membrane charge. Our results demonstrate the significant influence of membrane linkage as a straightforward biophysical parameter governing protein pattern formation. Our research points towards a simple yet intriguing mechanism by which cells can adeptly tune and switch protein patterns on the mesoscale.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Controlled Protein‐Membrane Interactions Modulate Self‐Organization of Min Protein Patterns ; volume:63 ; number:42 ; year:2024 ; extent:5
Angewandte Chemie / International edition. International edition ; 63, Heft 42 (2024) (gesamt 5)
- Creator
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Hasani, Mergime
Esch, Katharina
Zieske, Katja
- DOI
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10.1002/anie.202405046
- URN
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urn:nbn:de:101:1-2410071422390.626457208135
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:32 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Hasani, Mergime
- Esch, Katharina
- Zieske, Katja
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