Specialization of actin isoforms derived from the loss of key interactions with regulatory factors
Abstract: A paradox of eukaryotic cells is that while some species assemble a complex actin cytoskeleton from a single ortholog, other species utilize a greater diversity of actin isoforms. The physiological consequences of using different actin isoforms, and the molecular mechanisms by which highly conserved actin isoforms are segregated into distinct networks, are poorly known. Here, we sought to understand how a simple biological system, composed of a unique actin and a limited set of actin‐binding proteins, reacts to a switch to heterologous actin expression. Using yeast as a model system and biomimetic assays, we show that such perturbation causes drastic reorganization of the actin cytoskeleton. Our results indicate that defective interaction of a heterologous actin for important regulators of actin assembly limits certain actin assembly pathways while reinforcing others. Expression of two heterologous actin variants, each specialized in assembling a different network, rescues cytoskeletal organization and confers resistance to external perturbation. Hence, while species using a unique actin have homeostatic actin networks, actin assembly pathways in species using several actin isoforms may act more independently.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Specialization of actin isoforms derived from the loss of key interactions with regulatory factors ; day:18 ; month:02 ; year:2022 ; extent:28
The EMBO journal / European Molecular Biology Organization ; (18.02.2022) (gesamt 28)
- Urheber
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Boiero Sanders, Micaela
Toret, Christopher P.
Guillotin, Audrey
Antkowiak, Adrien
Vannier, Thomas
Robinson, Robert C.
Michelot, Alphée
- DOI
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10.15252/embj.2021107982
- URN
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urn:nbn:de:101:1-2022021814081248069605
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:33 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Boiero Sanders, Micaela
- Toret, Christopher P.
- Guillotin, Audrey
- Antkowiak, Adrien
- Vannier, Thomas
- Robinson, Robert C.
- Michelot, Alphée
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