Specialization of actin isoforms derived from the loss of key interactions with regulatory factors
Abstract: A paradox of eukaryotic cells is that while some species assemble a complex actin cytoskeleton from a single ortholog, other species utilize a greater diversity of actin isoforms. The physiological consequences of using different actin isoforms, and the molecular mechanisms by which highly conserved actin isoforms are segregated into distinct networks, are poorly known. Here, we sought to understand how a simple biological system, composed of a unique actin and a limited set of actin‐binding proteins, reacts to a switch to heterologous actin expression. Using yeast as a model system and biomimetic assays, we show that such perturbation causes drastic reorganization of the actin cytoskeleton. Our results indicate that defective interaction of a heterologous actin for important regulators of actin assembly limits certain actin assembly pathways while reinforcing others. Expression of two heterologous actin variants, each specialized in assembling a different network, rescues cytoskeletal organization and confers resistance to external perturbation. Hence, while species using a unique actin have homeostatic actin networks, actin assembly pathways in species using several actin isoforms may act more independently.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Specialization of actin isoforms derived from the loss of key interactions with regulatory factors ; day:18 ; month:02 ; year:2022 ; extent:28
The EMBO journal / European Molecular Biology Organization ; (18.02.2022) (gesamt 28)
- Creator
-
Boiero Sanders, Micaela
Toret, Christopher P.
Guillotin, Audrey
Antkowiak, Adrien
Vannier, Thomas
Robinson, Robert C.
Michelot, Alphée
- DOI
-
10.15252/embj.2021107982
- URN
-
urn:nbn:de:101:1-2022021814081248069605
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
16.08.2025, 4:33 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Boiero Sanders, Micaela
- Toret, Christopher P.
- Guillotin, Audrey
- Antkowiak, Adrien
- Vannier, Thomas
- Robinson, Robert C.
- Michelot, Alphée
Other Objects (12)
The role of actin isoforms in somatic embryogenesis in Norway spruce
Seventeen a-subunit isoforms of Paramecium V-ATPaseprovide high specialization in localization and function
Actin nucleator Spire 1 is a regulator of ectoplasmic specialization in the testis
Functional specialization of retinal Müller cell endfeet depends on an interplay between two syntrophin isoforms
Cytosolic actin isoforms form networks with different rheological properties that indicate specific biological function
Actin Regulatory Proteins in Dendritic Arborisation Neurons of Drosophila
Actin organization at intracellular membranes : targeting and regulatory interactions of the spir/formin actin nucleator complex
Gene regulatory network rewiring by disease variants and transcription factor isoforms
Alternative splicing is not a key source of chemerin isoforms diversity
Author Correction: Cytosolic actin isoforms form networks with different rheological properties that indicate specific biological function
p63 in health and disease: novel regulatory elements in isoforms and mutants
Actin-dependent mechanosensing in endothelial cells : regulatory aspects and targeting potential
The role of actin isoforms in somatic embryogenesis in Norway spruce
Seventeen a-subunit isoforms of Paramecium V-ATPaseprovide high specialization in localization and function
Actin nucleator Spire 1 is a regulator of ectoplasmic specialization in the testis
Functional specialization of retinal Müller cell endfeet depends on an interplay between two syntrophin isoforms
Cytosolic actin isoforms form networks with different rheological properties that indicate specific biological function
Actin Regulatory Proteins in Dendritic Arborisation Neurons of Drosophila
Actin organization at intracellular membranes : targeting and regulatory interactions of the spir/formin actin nucleator complex
Gene regulatory network rewiring by disease variants and transcription factor isoforms
Alternative splicing is not a key source of chemerin isoforms diversity
Author Correction: Cytosolic actin isoforms form networks with different rheological properties that indicate specific biological function
p63 in health and disease: novel regulatory elements in isoforms and mutants
Actin-dependent mechanosensing in endothelial cells : regulatory aspects and targeting potential
The role of actin isoforms in somatic embryogenesis in Norway spruce
Seventeen a-subunit isoforms of Paramecium V-ATPaseprovide high specialization in localization and function
Actin nucleator Spire 1 is a regulator of ectoplasmic specialization in the testis
Functional specialization of retinal Müller cell endfeet depends on an interplay between two syntrophin isoforms
Cytosolic actin isoforms form networks with different rheological properties that indicate specific biological function
Actin Regulatory Proteins in Dendritic Arborisation Neurons of Drosophila
Actin organization at intracellular membranes : targeting and regulatory interactions of the spir/formin actin nucleator complex
Gene regulatory network rewiring by disease variants and transcription factor isoforms
Alternative splicing is not a key source of chemerin isoforms diversity
Author Correction: Cytosolic actin isoforms form networks with different rheological properties that indicate specific biological function
p63 in health and disease: novel regulatory elements in isoforms and mutants