A novel domain regulating degradation of the glomerular slit diaphragm protein podocin in cell culture

Abstract: Mutations in the gene NPHS2 are the most common cause of hereditary steroid-resistant nephrotic syndrome. Its gene product, the stomatin family member protein podocin represents a core component of the slit diaphragm, a unique structure that bridges the space between adjacent podocyte foot processes in the kidney glomerulus. Dislocation and misexpression of slit diaphragm components have been described in the pathogenesis of acquired and hereditary nephrotic syndrome. However, little is known about mechanisms regulating cellular trafficking and turnover of podocin. Here, we discover a three amino acids-comprising motif regulating intracellular localization of podocin in cell culture systems. Mutations of this motif led to markedly reduced degradation of podocin. These findings give novel insight into the molecular biology of the slit diaphragm protein podocin, enabling future research to establish the biological relevance of podocin turnover and localization

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: PLoS one. 8, 2 (2013), e57078, DOI 10.1371/journal.pone.0057078, issn: 1932-6203 ; IN COPYRIGHT http://rightsstatements.org/page/InC/1.0 rs

Classification and Topics

• Classification: Biowissenschaften, Biologie

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2013
• Creator: Gödel, Markus ; Ostendorf, Benjamin Nils ; Baumer, Jessica Lea ; Weber, Katrin ; Huber, Tobias
• Contributor: BIOSS Centre for Biological Signalling Studies ; Klinik für Innere Medizin IV ; Fakultät für Biologie ; Medizinische Fakultät ; Albert-Ludwigs-Universität Freiburg

Further information

• DOI: 10.1371/journal.pone.0057078
• URN: urn:nbn:de:bsz:25-freidok-119222
• Rights: Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 04.04.2025, 9:46 AM CEST