Hochschulschrift
Nucleotide exchange and excision technology (NExT) DNA shuffling And Mutational studies on chloramphenicol actetyltransferase I (CATI)
Abstract: Overall stability is the key to application of proteins in technology and therapy. This thesis wants to analyze how structural perturbation by truncation in combination with directed evolution with selection only for activity and subsequent removal of the structural perturbation improves not only activity but also thermal and chemical stability, and even surprisingly positively influences the aggregation behavior.Furthermore this thesis wanted to investigate whether an already thermal and chemical stable enzyme with a two substrate mechanism and finely tuned interaction between subunits during catalysis can be further stabilized without the requirement of structural information or further hyperthermophilic homologs by structural perturbation and compensation strategy
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Anmerkungen
-
cc_by_nc http://creativecommons.org/licenses/by-nc/4.0/deed.de cc
Albert-Ludwigs-Universität Freiburg, Dissertation, 2007
- Schlagwort
-
DNA-Shuffling
Molekulare Evolution
Escherichia coli
- Ereignis
-
Veröffentlichung
- (wo)
-
Freiburg
- (wer)
-
Universität
- (wann)
-
2007
- Urheber
- Beteiligte Personen und Organisationen
-
Sippel, Albrecht E.
Müller, Kristian Mark
Fakultät für Biologie
Institut für Biologie III
Albert-Ludwigs-Universität Freiburg
- DOI
-
10.6094/UNIFR/11610
- URN
-
urn:nbn:de:bsz:25-freidok-116101
- Rechteinformation
-
Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
25.03.2025, 13:55 MEZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Objekttyp
- Hochschulschrift
Beteiligte
- Stebel, Sabine
- Sippel, Albrecht E.
- Müller, Kristian Mark
- Fakultät für Biologie
- Institut für Biologie III
- Albert-Ludwigs-Universität Freiburg
- Universität
Entstanden
- 2007
Ähnliche Objekte (12)
Structural and functional characterization of nucleotide excision repair proteins
Nucleotide excision repair pathway modulating both cancer risk and therapy
Nucleotide excision repair: interplay between nuclear compartmentalization, histone modifications and signaling
Structure-function analysis of human nucleotide excision DNA repair
Functions of RING1B and ZRF1 in ubiquitin-mediated regulation of nucleotide excision repair
Nucleotide Excision Repair : From Recognition to Incision of damaged DNA
Characterization of the multifunctional XPG protein during Nucleotide-excision-repair
Calorie restriction affects human nucleotide excision repair and the expression of aging-associated genes
Structural and functional characterization of nucleotide excision repair proteins : = Strukturelle und funktionelle Charakterisierung von Nucleotid-Exzisions-Reparatur-Proteinen
The Role of Poly(ADP-Ribosyl)ation in the Molecular and Cellular Response to Nucleotide Excision Repair DNA-Lesions
L'excision?...
Adult‐onset neurodegeneration in nucleotide excision repair disorders (NERDND): time to move beyond the skin
Structural and functional characterization of nucleotide excision repair proteins
Nucleotide excision repair pathway modulating both cancer risk and therapy
Nucleotide excision repair: interplay between nuclear compartmentalization, histone modifications and signaling
Structure-function analysis of human nucleotide excision DNA repair
Functions of RING1B and ZRF1 in ubiquitin-mediated regulation of nucleotide excision repair
Nucleotide Excision Repair : From Recognition to Incision of damaged DNA
Characterization of the multifunctional XPG protein during Nucleotide-excision-repair
Calorie restriction affects human nucleotide excision repair and the expression of aging-associated genes
Structural and functional characterization of nucleotide excision repair proteins : = Strukturelle und funktionelle Charakterisierung von Nucleotid-Exzisions-Reparatur-Proteinen
The Role of Poly(ADP-Ribosyl)ation in the Molecular and Cellular Response to Nucleotide Excision Repair DNA-Lesions
L'excision?...
Adult‐onset neurodegeneration in nucleotide excision repair disorders (NERDND): time to move beyond the skin
Structural and functional characterization of nucleotide excision repair proteins
Nucleotide excision repair pathway modulating both cancer risk and therapy
Nucleotide excision repair: interplay between nuclear compartmentalization, histone modifications and signaling
Structure-function analysis of human nucleotide excision DNA repair
Functions of RING1B and ZRF1 in ubiquitin-mediated regulation of nucleotide excision repair
Nucleotide Excision Repair : From Recognition to Incision of damaged DNA
Characterization of the multifunctional XPG protein during Nucleotide-excision-repair
Calorie restriction affects human nucleotide excision repair and the expression of aging-associated genes
Structural and functional characterization of nucleotide excision repair proteins : = Strukturelle und funktionelle Charakterisierung von Nucleotid-Exzisions-Reparatur-Proteinen
The Role of Poly(ADP-Ribosyl)ation in the Molecular and Cellular Response to Nucleotide Excision Repair DNA-Lesions
L'excision?...