Massively Parallel Double‐Selection Workflow for the Evolution of Molecular Switches Based on Surface‐Display in Escherichia coli
Abstract: Microbial surface display of proteins is a versatile method for a wide range of biotechnological applications. Herein, the use of a surface display system in E. coli for the evolution of a riboswitch from an RNA aptamer is presented. To this end, a streptavidin‐binding peptide (SBP) is displayed at the bacterial surface, which can be used for massively parallel selection using a magnetic separation system. Coupling gene expression from a riboswitch library to the display of SBP hence allows selection of library members that express strongly in the presence of a ligand. As excessive SBP expression leads to bacterial growth inhibition, it can be used to negatively select against leaky riboswitches expressing in the absence of ligand. Based on this principle, we devise a double selection workflow that enables quick selection of functional riboswitches with a comparatively low screening workload. The efficiency of our protocol by re‐discovering a previously isolated theophylline riboswitch from a library was demonstrated, as well as a new riboswitch that is similar in performance, but slightly more responsive at low theophylline concentrations. Our workflow is massively parallel and can be applied to the screening or pre‐screening of large molecular libraries.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Massively Parallel Double‐Selection Workflow for the Evolution of Molecular Switches Based on Surface‐Display in Escherichia coli ; day:11 ; month:05 ; year:2023 ; extent:12
Chemistry - a European journal ; (11.05.2023) (gesamt 12)
- Creator
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Givelet, Louis
Bienau, Alexandra
Simmel, Friedrich C.
- DOI
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10.1002/chem.202300845
- URN
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urn:nbn:de:101:1-2023051115504880859123
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:53 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Givelet, Louis
- Bienau, Alexandra
- Simmel, Friedrich C.
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