Reversible Self‐Assembly of Nucleic Acids in a Diffusiophoretic Trap

Abstract: The formation and dissociation of duplexes or higher order structures from nucleic acid strands is a fundamental process with widespread applications in biochemistry and nanotechnology. Here, we introduce a simple experimental system—a diffusiophoretic trap—for the non‐equilibrium self‐assembly of nucleic acid structures that uses an electrolyte gradient as the driving force. DNA strands can be concentrated up to hundredfold by a diffusiophoretic trapping force that is caused by the electric field generated by the electrolyte gradient. We present a simple equation for the field to guide selection of appropriate trapping electrolytes. Experiments with carboxylated silica particles demonstrate that the diffusiophoretic force is long‐ranged, extending over hundreds of micrometers. As an application, we explore the reversible self‐assembly of branched DNA nanostructures in the trap into a macroscopic gel. The structures assemble in the presence of an electrolyte gradient, and disassemble upon its removal, representing a prototypical adaptive response to a macroscopic non‐equilibrium state.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Reversible Self‐Assembly of Nucleic Acids in a Diffusiophoretic Trap ; day:05 ; month:03 ; year:2024 ; extent:7 ; Angewandte Chemie / International edition. International edition ; (05.03.2024) (gesamt 7)

Contributors, Places and Time

• Creator: Katzmeier, Florian ; Simmel, Friedrich C.

Further information

• DOI: 10.1002/anie.202317118
• URN: urn:nbn:de:101:1-2024030614194155896727
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 10:47 AM CEST