Point-of-care system for HTLV-1 proviral load quantification by digital mediator displacement LAMP
Abstract: This paper presents a universal point-of-care system for fully automated quantification of human T-cell lymphotropic virus type 1 (HTLV-1) proviral load, including genomic RNA, based on digital reverse RNA transcription and c-DNA amplification by MD LAMP (mediator displacement loop-mediated isothermal amplification). A disposable microfluidic LabDisk with pre-stored reagents performs automated nucleic acid extraction, reaction setup, emulsification, reverse transcription, digital DNA amplification, and quantitative fluorogenic endpoint detection with universal reporter molecules. Automated nucleic acid extraction from a suspension of HTLV-1-infected CD4+ T-lymphocytes (MT-2 cells) yielded 8 ± 7 viral nucleic acid copies per MT-2 cell, very similar to the manual reference extraction (7 ± 2 nucleic acid copies). Fully automated sample processing from whole blood spiked with MT-2 cells showed a comparable result of 7 ± 3 copies per MT-2 cell after a run time of two hours and 10 min
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Micromachines. - 12, 2 (2021) , 159, ISSN: 2072-666X
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2021
- Creator
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Becherer, Lisa
Hess, Jacob Friedrich
Frischmann, Sieghard
Bakheit, Mohammed
Nitschko, Hans
Stinco, Silvina
Zitz, Friedrich
Hofer, Hannes
Porro, Giampiero
Hausladen, Florian
Stock, Karl
Drossart, Dominik
Wurm, Holger
Kuhn, Hanna
Huber, Dominik
Hutzenlaub, Tobias
Paust, Nils
Keller, Mark
Strohmeier, Oliver
Wadle, Simon
Borst, Nadine
Zengerle, Roland
Stetten, Felix von
- DOI
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10.3390/mi12020159
- URN
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urn:nbn:de:bsz:25-freidok-1760273
- Rights
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Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:37 AM CEST
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Associated
- Becherer, Lisa
- Hess, Jacob Friedrich
- Frischmann, Sieghard
- Bakheit, Mohammed
- Nitschko, Hans
- Stinco, Silvina
- Zitz, Friedrich
- Hofer, Hannes
- Porro, Giampiero
- Hausladen, Florian
- Stock, Karl
- Drossart, Dominik
- Wurm, Holger
- Kuhn, Hanna
- Huber, Dominik
- Hutzenlaub, Tobias
- Paust, Nils
- Keller, Mark
- Strohmeier, Oliver
- Wadle, Simon
- Borst, Nadine
- Zengerle, Roland
- Stetten, Felix von
- Universität
Time of origin
- 2021
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