Metabolic characterization of directly reprogrammed renal tubular epithelial cells (iRECs)
Abstract: Fibroblasts can be directly reprogrammed to induced renal tubular epithelial cells (iRECs) using four transcription factors. These engineered cells may be used for disease modeling, cell replacement therapy or drug and toxicity testing. Direct reprogramming induces drastic changes in the transcriptional landscape, protein expression, morphological and functional properties of cells. However, how the metabolome is changed by reprogramming and to what degree it resembles the target cell type remains unknown. Using untargeted gas chromatography-mass spectrometry (GC-MS) and targeted liquid chromatography-MS, we characterized the metabolome of mouse embryonic fibroblasts (MEFs), iRECs, mIMCD-3 cells, and whole kidneys. Metabolic fingerprinting can distinguish each cell type reliably, revealing iRECs are most similar to mIMCD-3 cells and clearly separate from MEFs used for reprogramming. Treatment with the cytotoxic drug cisplatin induced typical changes in the metabolic profile of iRECs commonly occurring in acute renal injury. Interestingly, metabolites in the medium of iRECs, but not of mIMCD-3 cells or fibroblast could distinguish treated and non-treated cells by cluster analysis. In conclusion, direct reprogramming of fibroblasts into renal tubular epithelial cells strongly influences the metabolome of engineered cells, suggesting that metabolic profiling may aid in establishing iRECs as in vitro models for nephrotoxicity testing in the future
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Notes
-
Scientific reports. - 8, 1 (2018) , 3878, ISSN: 2045-2322
- Event
-
Veröffentlichung
- (where)
-
Freiburg
- (who)
-
Universität
- (when)
-
2019
- Creator
- Contributor
- DOI
-
10.1038/s41598-018-22073-7
- URN
-
urn:nbn:de:bsz:25-freidok-1477800
- Rights
-
Kein Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:19 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
Time of origin
- 2019
Other Objects (12)
Metabolic characterization of directly reprogrammed renal tubular epithelial cells (iRECs)
Funktionsaufnahme, Rejektionskrisen und Überlebensrate von Nierentransplantaten : retrospektive Auswertung von Daten eines Transplantationszentrums
Das Nephronophthisegen NPHP1 beeinflusst die Polarität von motilen Zilien im Xenopus laevis
Untersuchungen zur Funktion des Nephronophthise Proteins Inversin
The complex of BBS1 and NPHP7 is required for motility of cilia and is interacting physically and genetically : = BBS1 und NPHP7 interagieren und der Komplex ist notwendig für die Zilienmotilität im Zebrafisch
Autosomal dominante polyzystische Nierenerkrankung : der Transport von Polyzystin 2 wird phosphorylierungsabhängig von PACS-1 reguliert
Control of directed cell migration after tubular cell injury by nucleotide signaling
CMTM4 is a novel interactor of CXCR4 and regulator of CXCL12/CXCR4 signaling
Role of the transcription factors hic1, myca and the basement membrane in the zebrafish pronephros regeneration after laser induced injury
Histone deacetylases cooperate with nf-κb to support the immediate migratory response after zebrafish pronephros injury
Evaluation of deceased donor kidney transplantation in the eurotransplant senior program in comparison to standard allocation
FAT1 mutations cause a glomerulotubular nephropathy
Metabolic characterization of directly reprogrammed renal tubular epithelial cells (iRECs)
Funktionsaufnahme, Rejektionskrisen und Überlebensrate von Nierentransplantaten : retrospektive Auswertung von Daten eines Transplantationszentrums
Das Nephronophthisegen NPHP1 beeinflusst die Polarität von motilen Zilien im Xenopus laevis
Untersuchungen zur Funktion des Nephronophthise Proteins Inversin
The complex of BBS1 and NPHP7 is required for motility of cilia and is interacting physically and genetically : = BBS1 und NPHP7 interagieren und der Komplex ist notwendig für die Zilienmotilität im Zebrafisch
Autosomal dominante polyzystische Nierenerkrankung : der Transport von Polyzystin 2 wird phosphorylierungsabhängig von PACS-1 reguliert
Control of directed cell migration after tubular cell injury by nucleotide signaling
CMTM4 is a novel interactor of CXCR4 and regulator of CXCL12/CXCR4 signaling
Role of the transcription factors hic1, myca and the basement membrane in the zebrafish pronephros regeneration after laser induced injury
Histone deacetylases cooperate with nf-κb to support the immediate migratory response after zebrafish pronephros injury
Evaluation of deceased donor kidney transplantation in the eurotransplant senior program in comparison to standard allocation
FAT1 mutations cause a glomerulotubular nephropathy
Metabolic characterization of directly reprogrammed renal tubular epithelial cells (iRECs)
Funktionsaufnahme, Rejektionskrisen und Überlebensrate von Nierentransplantaten : retrospektive Auswertung von Daten eines Transplantationszentrums
Das Nephronophthisegen NPHP1 beeinflusst die Polarität von motilen Zilien im Xenopus laevis
Untersuchungen zur Funktion des Nephronophthise Proteins Inversin
The complex of BBS1 and NPHP7 is required for motility of cilia and is interacting physically and genetically : = BBS1 und NPHP7 interagieren und der Komplex ist notwendig für die Zilienmotilität im Zebrafisch
Autosomal dominante polyzystische Nierenerkrankung : der Transport von Polyzystin 2 wird phosphorylierungsabhängig von PACS-1 reguliert
Control of directed cell migration after tubular cell injury by nucleotide signaling
CMTM4 is a novel interactor of CXCR4 and regulator of CXCL12/CXCR4 signaling
Role of the transcription factors hic1, myca and the basement membrane in the zebrafish pronephros regeneration after laser induced injury
Histone deacetylases cooperate with nf-κb to support the immediate migratory response after zebrafish pronephros injury
Evaluation of deceased donor kidney transplantation in the eurotransplant senior program in comparison to standard allocation