Role of the transcription factors hic1, myca and the basement membrane in the zebrafish pronephros regeneration after laser induced injury

Abstract: Acute kidney injury (AKI) with the possible progression to chronic kidney disease is still a worldwide public health challenge. Although spontaneous recovery might take place, AKI is generally associated with poor outcome, since many of the affected patients remain dialysis-dependent. Therefore, the need for understanding the underlying pathophysiology and the regeneration process remains urgent. The laser-mediated ablation of collectively migrating tubular epithelial cells of the zebrafish pronephros represents a unique AKI model. In this system, transient injuries of the kidney tubule are repaired within hours via collective cell migration (CCM) of the neighbouring tubular epithelium. Here, I further characterize the molecular factors that connect CCM to tissue repair. Previous work has implicated the transcription factors hypermethylated in cancer 1 (hic1), myca in the regeneration process. Furthermore, the basement membrane is considered to be essential guiding matrix for the regenerating renal epithelia. My results show that the pronephros regeneration is not affected in genetic loss-of-function models of hic1 or its putative targets shroom1 and shroom3. Since no zebrafish mutant allele of myca was available, I used the CRISPR/Cas9 technology to generate novel null alleles for both myca and hic1. Surprisingly, preliminary data implies that intact basement membrane might not be required for pronephros regeneration after laser induced injury

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: Universität Freiburg, Dissertation, 2019

Classification and Topics

• Classification: Biowissenschaften, Biologie
• Keyword: Membrane, Basement ; Transcription factors ; Wolffian body ; Zebrabärbling ; Niere ; CRISPR/Cas-Methode ; Basalmembran ; Regeneration

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2019
• Creator: Wurditsch, Anne
• Contributor: Walz, Gerd ; Yakulov, Toma ; Albert-Ludwigs-Universität Freiburg. Medizinische Fakultät

Further information

• DOI: 10.6094/UNIFR/149383
• URN: urn:nbn:de:bsz:25-freidok-1493832
• Rights: Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 25.03.2025, 1:57 PM CET