Lineage tracing identifies in vitro microglia‐to‐neuron conversion by NeuroD1 expression
Abstract: Neuronal regeneration to replenish lost neurons after injury is critical for brain repair. Microglia, brain-resident macrophages that have the propensity to accumulate at the site of injury, can be a potential source for replenishing lost neurons through fate conversion into neurons, induced by forced expression of neuronal lineage-specific transcription factors. However, it has not been strictly demonstrated that microglia, rather than central nervous system-associated macrophages, such as meningeal macrophages, convert into neurons. Here, we show that NeuroD1-transduced microglia can be successfully converted into neurons in vitro using lineage-mapping strategies. We also found that a chemical cocktail treatment further promoted NeuroD1-induced microglia-to-neuron conversion. NeuroD1 with loss-of-function mutation, on the other hand, failed to induce the neuronal conversion. Our results indicate that microglia are indeed reprogrammed into neurons by NeuroD1 with neurogenic transcriptional activity
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Genes to cells. - 28, 7 (2023) , 526-534, ISSN: 1365-2443
- Classification
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Medizin, Gesundheit
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2023
- Creator
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Irie, Takashi
Matsuda‐Ito, Kanae
Matsuda, Taito
Masuda, Takahiro
Prinz, Marco
Isobe, Noriko
Nakashima, Kinichi
- DOI
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10.1111/gtc.13033
- URN
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urn:nbn:de:bsz:25-freidok-2359993
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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25.10.2070, 12:00 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Irie, Takashi
- Matsuda‐Ito, Kanae
- Matsuda, Taito
- Masuda, Takahiro
- Prinz, Marco
- Isobe, Noriko
- Nakashima, Kinichi
- Universität
Time of origin
- 2023
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