Hochschulschrift
Sites of transcription initiation drive mRNA isoform selection
Abstract: The generation of distinct messenger RNA isoforms through alternative RNA processing modulates the expression and function of genes, often in a cell-type-specific manner. Here, we assess the regulatory relationships between transcription initiation, alternative splicing, and 3′ end site selection. Applying long-read sequencing to accurately represent even the longest transcripts from end to end, we quantify mRNA isoforms in Drosophila tissues, including the transcriptionally complex nervous system. We find that in Drosophila heads, as well as in human cerebral organoids, 3′ end site choice is globally influenced by the site of transcription initiation (TSS). “Dominant promoters,” characterized by specific epigenetic signatures including p300/CBP binding, impose a transcriptional constraint to define splice and polyadenylation variants. In vivo deletion or overexpression of dominant promoters as well as p300/CBP loss disrupted the 3′ end expression landscape. Our study demonstrates the crucial impact of TSS choice on the regulation of transcript diversity and tissue identity
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Cell. - 186, 11 (2023) , 2438-2455.e22, ISSN: 1097-4172
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2024
- Creator
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Alfonso-Gonzalez, Carlos
Legnini, Ivano
Holec, Sarah
Arrigoni, Laura
Ozbulut, Hasan Can
Mateos, Fernando
Koppstein, David
Rybak-Wolf, Agnieszka
Bönisch, Ulrike
Rajewsky, Nikolaus
Hilgers, Valérie
- DOI
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10.1016/j.cell.2023.04.012
- URN
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urn:nbn:de:bsz:25-freidok-2458987
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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25.03.2025, 1:51 PM CET
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Object type
- Hochschulschrift
Associated
- Alfonso-Gonzalez, Carlos
- Legnini, Ivano
- Holec, Sarah
- Arrigoni, Laura
- Ozbulut, Hasan Can
- Mateos, Fernando
- Koppstein, David
- Rybak-Wolf, Agnieszka
- Bönisch, Ulrike
- Rajewsky, Nikolaus
- Hilgers, Valérie
- Universität
Time of origin
- 2024
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Sites of transcription initiation drive mRNA isoform selection
Characterization of a novel long FOXP3 mRNA isoform
Hematopoietic transcription factors and differential cofactor binding regulate PRKACB isoform expression
Functional interactions of the Transcription Factor B during transcription initiation in Pyrococcus furiosus
Statistics for transcription factor binding sites
Structural basis of human mitochondrial transcription initiation and processive elongation
Interplay of nucleosome positioning and transcription initiation in Schizosaccharomyces pombe
Single-molecule analysis of transcription initiation in archaea and eukaryotes
Über Mechanismen der Initiation, Elongation und Termination in der Transcription
RNA polymerase II–TFIIB structure and mechanism of transcription initiation
Co-occurrence of transcription factor binding sites
Isoform dependent oncogenic or tumor suppressive properties of the transcription factor MEIS2 in neuroblastoma
Sites of transcription initiation drive mRNA isoform selection
Characterization of a novel long FOXP3 mRNA isoform
Hematopoietic transcription factors and differential cofactor binding regulate PRKACB isoform expression
Functional interactions of the Transcription Factor B during transcription initiation in Pyrococcus furiosus
Statistics for transcription factor binding sites
Structural basis of human mitochondrial transcription initiation and processive elongation
Interplay of nucleosome positioning and transcription initiation in Schizosaccharomyces pombe
Single-molecule analysis of transcription initiation in archaea and eukaryotes
Über Mechanismen der Initiation, Elongation und Termination in der Transcription
RNA polymerase II–TFIIB structure and mechanism of transcription initiation
Co-occurrence of transcription factor binding sites