Impacts of long noncoding RNA MALAT1 on LPS-induced periodontitis via modulating miR-155/SIRT1 axis
Objectives: Periodontitis, a dental disease that causes inflammation of gums is triggered by a bacterial infection. Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (lncRNA MALAT1) has been reported to participate in inflammatory diseases. In the present study, we investigated the effects of lncRNA MALAT1 on periodontal ligament cells (PDLCs) with lipopolysaccharide (LPS) induction. Methods: MALAT1, microRNA-155 (miR-155), Sirtuin 1 (SIRT1), Bax, and Bcl-2 RNA expressions were detected by using real-time quantitative polymerase chain reaction (RT-qPCR). PDLC viability and apoptosis were assessed by using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium salt (MTT) assay and flow cytometry. Enzyme-linked immunosorbent assay (ELISA) assessed secretions of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). Luciferase reporter test was applied for confirming the binding of miR-155 with MALAT1 and SIRT1, respectively. Results: Overexpression of MALAT1 facilitated the LPS-induced periodontitis while MALAT1 suppression restrained LPS-induced injury. Also, miR-155 was expressed in LPS-induced PDLCs. Moreover, MALAT1 negatively regulated miR-155 followed by the up-regulation of SIRT1 which in turn reduced the inflammation in LPS-induced PDLCs. Conclusions: We concluded that upregulated lncRNA MALAT1 could accelerate periodontitis through the regulation of miR-155/SIRT1. Our findings suggested a novel MALAT1/miR-155/SIRT1 pathway for the treatment of periodontitis.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Impacts of long noncoding RNA MALAT1 on LPS-induced periodontitis via modulating miR-155/SIRT1 axis ; volume:47 ; number:5 ; year:2022 ; pages:595-601 ; extent:07
Turkish journal of biochemistry ; 47, Heft 5 (2022), 595-601 (gesamt 07)
- Creator
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Hu, Wanjun
Lei, Xiubing
Luo, Jianglong
Gou, Xing
- DOI
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10.1515/tjb-2022-0077
- URN
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urn:nbn:de:101:1-2022103013024813109504
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:24 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Hu, Wanjun
- Lei, Xiubing
- Luo, Jianglong
- Gou, Xing
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Retraction Note: Protective functions of myricetin in LPS-induced cardiomyocytes H9c2 cells injury by regulation of MALAT1
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Protective functions of myricetin in LPS-induced cardiomyocytes H9c2 cells injury by regulation of MALAT1
Sirt1 regulates testosterone biosynthesis in Leydig cells via modulating autophagy
LncRNA MALAT1 promotes osteoarthritis by modulating miR-150-5p/AKT3 axis
Inhibitory effect of naringenin on LPS-induced skin senescence by SIRT1 regulation in HDFs
Retraction Note: Protective functions of myricetin in LPS-induced cardiomyocytes H9c2 cells injury by regulation of MALAT1
Emodin alleviates cholestatic liver injury by modulating Sirt1/Fxr signaling pathways
Flotillins affect LPS-induced TLR4 signaling by modulating the trafficking and abundance of CD14
Ergosterol Attenuates LPS-Induced Myocardial Injury by Modulating Oxidative Stress and Apoptosis in Rats
Meteorin-like/Meteorin-β protects LPS-induced acute lung injury by activating SIRT1-P53-SLC7A11 mediated ferroptosis pathway
Long non-coding RNA MALAT1 drives gastric cancer progression by regulating HMGB2 modulating the miR-1297
Correction: Flotillins affect LPS-induced TLR4 signaling by modulating the trafficking and abundance of CD14
Ginkgetin aglycone ameliorates LPS-induced acute kidney injury by activating SIRT1 via inhibiting the NF-κB signaling pathway
Protective functions of myricetin in LPS-induced cardiomyocytes H9c2 cells injury by regulation of MALAT1
Sirt1 regulates testosterone biosynthesis in Leydig cells via modulating autophagy
LncRNA MALAT1 promotes osteoarthritis by modulating miR-150-5p/AKT3 axis
Inhibitory effect of naringenin on LPS-induced skin senescence by SIRT1 regulation in HDFs
Retraction Note: Protective functions of myricetin in LPS-induced cardiomyocytes H9c2 cells injury by regulation of MALAT1
Emodin alleviates cholestatic liver injury by modulating Sirt1/Fxr signaling pathways
Flotillins affect LPS-induced TLR4 signaling by modulating the trafficking and abundance of CD14
Ergosterol Attenuates LPS-Induced Myocardial Injury by Modulating Oxidative Stress and Apoptosis in Rats
Meteorin-like/Meteorin-β protects LPS-induced acute lung injury by activating SIRT1-P53-SLC7A11 mediated ferroptosis pathway
Long non-coding RNA MALAT1 drives gastric cancer progression by regulating HMGB2 modulating the miR-1297
Correction: Flotillins affect LPS-induced TLR4 signaling by modulating the trafficking and abundance of CD14