A mechanism of cohesin‐dependent loop extrusion organizes zygotic genome architecture
Abstract: Fertilization triggers assembly of higher‐order chromatin structure from a condensed maternal and a naïve paternal genome to generate a totipotent embryo. Chromatin loops and domains have been detected in mouse zygotes by single‐nucleus Hi‐C (snHi‐C), but not bulk Hi‐C. It is therefore unclear when and how embryonic chromatin conformations are assembled. Here, we investigated whether a mechanism of cohesin‐dependent loop extrusion generates higher‐order chromatin structures within the one‐cell embryo. Using snHi‐C of mouse knockout embryos, we demonstrate that the zygotic genome folds into loops and domains that critically depend on Scc1‐cohesin and that are regulated in size and linear density by Wapl. Remarkably, we discovered distinct effects on maternal and paternal chromatin loop sizes, likely reflecting differences in loop extrusion dynamics and epigenetic reprogramming. Dynamic polymer models of chromosomes reproduce changes in snHi‐C, suggesting a mechanism where cohesin locally compacts chromatin by active loop extrusion, whose processivity is controlled by Wapl. Our simulations and experimental data provide evidence that cohesin‐dependent loop extrusion organizes mammalian genomes over multiple scales from the one‐cell embryo onward.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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A mechanism of cohesin‐dependent loop extrusion organizes zygotic genome architecture ; volume:36 ; number:24 ; year:2017 ; pages:3600-3618 ; extent:19
The EMBO journal / European Molecular Biology Organization ; 36, Heft 24 (2017), 3600-3618 (gesamt 19)
- Creator
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Gassler, Johanna
Brandão, Hugo B.
Imakaev, Maxim
Flyamer, Ilya M.
Ladstätter, Sabrina
Bickmore, Wendy A.
Peters, Jan-Michael
Mirny, Leonid A.
Tachibana, Kikuë
- DOI
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10.15252/embj.201798083
- URN
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urn:nbn:de:101:1-2022100306420178113446
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:25 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Gassler, Johanna
- Brandão, Hugo B.
- Imakaev, Maxim
- Flyamer, Ilya M.
- Ladstätter, Sabrina
- Bickmore, Wendy A.
- Peters, Jan-Michael
- Mirny, Leonid A.
- Tachibana, Kikuë
Other Objects (12)
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Cohesin distribution alone predicts chromatin organization in yeast via conserved-current loop extrusion
Sensitivity of cohesin–chromatin association to high-salt treatment corroborates non-topological mode of loop extrusion
The cohesin acetylation cycle controls chromatin loop length through a PDS5A brake mechanism
Different NIPBL requirements of cohesin-STAG1 and cohesin-STAG2
Super-resolution visualization and modeling of human chromosomal regions reveals cohesin-dependent loop structures
A new meiosis‐specific cohesin complex implicated in the cohesin code for homologous pairing
MoDLE: high-performance stochastic modeling of DNA loop extrusion interactions
Closed‐Loop Recyclable and Extrusion Reprocessable Thermosets Enabled by Guanylthiourea Structure
Cohesin prevents cross-domain gene coactivation
Cohesin biology meets the loop extrusion model
Cell cycle-specific DNA loop extrusion by cohesin and condensin
Cohesin‐mediated DNA loop extrusion resolves sister chromatids in G2 phase
Cohesin distribution alone predicts chromatin organization in yeast via conserved-current loop extrusion
Sensitivity of cohesin–chromatin association to high-salt treatment corroborates non-topological mode of loop extrusion
The cohesin acetylation cycle controls chromatin loop length through a PDS5A brake mechanism
Different NIPBL requirements of cohesin-STAG1 and cohesin-STAG2
Super-resolution visualization and modeling of human chromosomal regions reveals cohesin-dependent loop structures
A new meiosis‐specific cohesin complex implicated in the cohesin code for homologous pairing
MoDLE: high-performance stochastic modeling of DNA loop extrusion interactions
Closed‐Loop Recyclable and Extrusion Reprocessable Thermosets Enabled by Guanylthiourea Structure
Cohesin prevents cross-domain gene coactivation
Cohesin biology meets the loop extrusion model
Cell cycle-specific DNA loop extrusion by cohesin and condensin
Cohesin‐mediated DNA loop extrusion resolves sister chromatids in G2 phase
Cohesin distribution alone predicts chromatin organization in yeast via conserved-current loop extrusion
Sensitivity of cohesin–chromatin association to high-salt treatment corroborates non-topological mode of loop extrusion
The cohesin acetylation cycle controls chromatin loop length through a PDS5A brake mechanism
Different NIPBL requirements of cohesin-STAG1 and cohesin-STAG2
Super-resolution visualization and modeling of human chromosomal regions reveals cohesin-dependent loop structures
A new meiosis‐specific cohesin complex implicated in the cohesin code for homologous pairing
MoDLE: high-performance stochastic modeling of DNA loop extrusion interactions
Closed‐Loop Recyclable and Extrusion Reprocessable Thermosets Enabled by Guanylthiourea Structure