An Acid‐Activatable Fluorescence Probe for Imaging Osteocytic Bone Resorption Activity in Deep Bone Cavities
Abstract: A rationally designed pH‐activatable fluorescent probe (pHocas‐RIS) has been used to measure localised pH levels in osteocytic lacunae in bone tissue. Conjugation of the moderate bone‐binding drug risedronate to a pH‐activatable BODIPY fluorophore enables the probe to penetrate osteocytic lacunae cavities that are embedded deep within the bone matrix. After injection of pHocas‐RIS, any osteocytic lacunae caused by bone‐resorbing osteocytes cause the probe to fluoresce in vivo, thus allowing imaging by intravital two‐photon excitation microscopy. This pH responsive probe enabled the visualization of the bone mineralizing activities of acid producing osteocytes in real time, thus allowing the study of their central role in remodeling the bone‐matrix in healthy and disease states.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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An Acid‐Activatable Fluorescence Probe for Imaging Osteocytic Bone Resorption Activity in Deep Bone Cavities ; volume:59 ; number:47 ; year:2020 ; pages:20996-21000 ; extent:5
Angewandte Chemie / International edition. International edition ; 59, Heft 47 (2020), 20996-21000 (gesamt 5)
- Creator
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Hashimoto, Ryu
Minoshima, Masafumi
Kikuta, Junichi
Yari, Shinya
Bull, Steven D.
Ishii, Masaru
Kikuchi, Kazuya
- DOI
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10.1002/anie.202006388
- URN
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urn:nbn:de:101:1-2022061314190826635409
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:35 AM CEST
Data provider
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Associated
- Hashimoto, Ryu
- Minoshima, Masafumi
- Kikuta, Junichi
- Yari, Shinya
- Bull, Steven D.
- Ishii, Masaru
- Kikuchi, Kazuya
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